Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.1594.1599Mentha longifolia (L.) Leaf Extract in Rat]]>Gharib NaseriMohammad Kazem Gharib NaseriZahra MohammadianMaryam Omidi BirganiMarzie 1220081112The effect of Mentha longifolia (L.) leaf hydroalcoholic
extract (MLE) was examined on rat ileal smooth muscle contractions. Last
portion of ileum from male adult Wistar rat was mounted in an organ bath
containing Tyrode solution. The tissue was contracted by carbachol (CCh,
10 μM), KCl (60 mM) and BaCl2 (4 mM) and then MLE (0.0625-1
mg mL-1) was added to the bath cumulatively. The effect of
MLE on KCl-induced contraction was examined after tissue incubation with
propranolol (1 μM), naloxone (1 μM) and Nω-nitro-L-arginine
methyl ester (L-NAME, 100 μM). The effect of MLE on CaCl2-induced
ileal contraction in Ca2+-free with high potassium Tyrode solution
was also evaluated. The role of potassium channels was examined by ileum
incubation (5 min) with tetraethylammonium (TEA, 1 mM). The results showed
that KCl-, CCh and BaCl2-induced ileal contractions were inhibited
(p<0.001) by cumulative concentrations of MLE with the same potency.
In addition, MLE (0.25-1 mg mL-1) inhibited (p<0.01) ileal
contractions induced by CaCl2 (0.45-2.7 mM) in a concentration-related
manner. The antispasmodic effect of MLE was affected neither by propranolol,
L-NAME nor by naloxone. The MLE concentration-response curve was shifted
to the right (p<0.05) by tissue incubation with TEA. From results it
may be suggested that Mentha longifolia hydroalcoholic leaf extract
induces its spasmolytic activity mainly through disturbance in calcium
mobilization and partly by potassium channels activation. Present results
show that Mentha longifolia leaf extract exerts relaxant effects
on intestinal smooth muscle, consistent with the traditional use of the
plant to treat gastrointestinal disorders such as diarrhea and colic.]]>Abou-Jawdah, Y., H. Sobh and A. Salameh,20025032083213Amzazi, S., S. Ghoulami, Y. Bakri, A. Idrissi, S.W. Fkil-Tetouani and A. Benjouad,2003Mentha longifolia.]]>58531534Bolton, T.B.,197959606718Borrelli, F., F. Capasso, R. Capasso, V. Ascione, G. Aviello, R. Longo and A.A. Izzo,2006Boswellia serrata on intestinal motility in rodents: Inhibition of diarrhoea without constipation.]]>148553560Brown, K.J. and R.J. Summers,20011 and β3-adrenoceptor mediated smooth muscle relaxation in hypothyroid rat ileum.]]>415257263El-Bardai, S., M.C. Hamaide, B. Lyoussi, J. Quetin-Leclercq, N. Morel and M. Wibo,2004492269272Fujimoto, S. and M. Mori,2004487175182Ghoulami, S.A., I.L. Idrissi and S. Fkih-Tetouani,2001Mentha longifolia of Morocco.]]>72596598Gilani, A.H., S. Bashir, K.H. Janbaz and A.J. Shah, 2005Hibiscus rosasinensis in constipation and diarrhoea.]]>102289294Gilani, A.H., A.U. Khan, M. Raoof, M.N. Ghayur, B.S. Siddiqui, W. Vohra and S. Begum,2008Hyoscyamus niger are mediated through dual blockade of muscarinic receptors and Ca2+ channels.]]>228799Gray, A.C., P.J. White and I.M. Coupar,2005144687694Guedes, D.N., D.F. Silva, J.M. Barbosa-Filho and I.A. Medeiros,20043718811887Izzo, A.A., N. Mascolo and F. Capasso,1998124825831Jang, G.J., D.S. Ahn, Y.E. Cho, K.G. Morgan and Y.H. Lee,20052-ceramide induces vasodilation in phenylephrine-induced pre-contracted rat thoracic aorta: Role of RhoA/Rho-kinase and intracellular Ca2+ concentration. ]]>372242250Kanada, A., F. Hata, N. Suthamnatpong, T. Maehara, T. Ishii, T. Takeuchi and O. Yagasaki,1992216287292Kaya, T.T., G. Kokhan, A.S. Soydan, M. Arpacik and B. Karadas,2002442147153Khan, S.W. and S. Khatoon,2008404358Kozan, E., E. Kupeli and E. Yesilada,2006in vivo anthelmintic activity.]]>108211216Latif, A., Z.K. Shinwari, J. Hussain and S. Murtaza,2006111521Liu, S., H.Z. Hu, J. Ren, C. Gao, N. Gao, Z. Lin, Y. Xia and J.D. Wood,2001in vitro.]]>281G237G246Madeira, S.V.F., F.J.A. Matos and D.C. Leal-Criddle,2002Ocimum gratissimum on isolated ileum of the guinea pig.]]>8114Mimica-Dukic, N., B. Bozin, M. Sokovic, B. Mihajlovic and M. Matavulj,2003Mentha species essential oils.]]>69413419Ozaki, M., T. Nagatomo, T. Maeda, S. Kishioka and H. Yamamoto,20062913491354Shaiq Ali, M., M. Saleem, W. Ahmad, M. Parvez and R. Yamdagni,2002Mentha longifolia (Lamiaceae).]]>59889895Ali, M.S., W. Ahmed, M. Saleem and T. Khan,2006Mentha longifolia (Lamiaceae).]]>20953960Shahverdi, A.R., F. Rafii, F. Tavassoli, M. Bagheri, F. Attar and A. Ghahraman,2004Mentha longifolia var. chorodictya Rech F. reduces the nitrofurantoin resistance of strains of enterobacteriaceae.]]>18911914Sousa, P.J., P.J. Magalhaes, C.C. Lima, V.S. Oliveira and J.H. Leal-Cardoso,199730787791Tanovic, A., M. Jimenez and E. Fernandez,200067531541Zhang, W.W., Y. Li, X.Q. Wang, F. Tian, H. Cao, M.W. Wang and Q.S. Sun,20051144144418