Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.1085.1092Escherichia coli O157
and O157:H7 Strains Isolated from Sheep Carcasses in Shiraz-Iran]]>ShekarforoushShahram TahamtanYahya PourbakhshAli 82008118Enterohaemorrhagic Escherichia coli constitute a subset of
serotypes (E. coli O157 and some other serogroups) of Shiga toxin-producing
E. coli firmly associated with severe human illnesses like bloody
diarrhoea and haemolytic uraemic syndrome. Escherichia coli O157:H7
is a zoonotic pathogen. They rarely cause disease in animals, live in
the intestines of healthy sheep and ruminants are recognized as their
main natural reservoir, so they can contaminate meat during slaughtering
practices. The purpose of this study was epidemiological survey on the
occurrence of E. coli O157:H7 in healthy sheep in Shiraz-Iran.
Polymerase Chain Reaction (PCR) assay was developed to detect the Stx2
gene the only bacterial factor that has been associated with more
severe disease. During a period of 7 months (December 2005 to June 2006),
153 slaughtered sheep at Shiraz slaughterhouse, were randomly selected
and examined for surface carriage of E. coli O157:H7 by conventional
plating and Stx2 gene detection by PCR technique. E. coli
O157:H7 was found in 6(3.92%) of 153 sheep. The bacteria were isolated
from 5(3.34%) of 114 and 1(2.63%) of 38 sheep two or under two and more
than 2 years old, respectively (p = 0.5). The contamination rate might
vary depending on season, age and infection time. The higher frequency
for younger animals may be due to differences in the composition of the
gastrointestinal flora resulting from differences in diet. This is the
first report of the presence of E. coli O157:H7 in sheep from Iran.]]>Armstrong, G.L., J. Hollingsworth and J.G. Morris,1996Escherichia coli O157:H7 as a model of entry of a new pathogen into the food supply of the developed world.]]>182951Aslantas, O., S. Erdogan, Z. Cantekin, I. Gulacti and G.A. Evrendilek,2006Escherichia coli O157 from Turkish cattle.]]>106338342Beutin, L., D. Geier, H. Steinruck, S. Zimmermann and F. Scheutz,1993Escherichia coli in seven different species of healthy domestic animals.]]>3124832488Bolton, D.J., A.M. Doherty and J.J. Sheridan,200166119129Bonardi, S., E. Maggi, A. Bottarelli, M.L. Pacciarini and A. Ansuini et al.,1999Escherichia coli O157:H7 from cattle at slaughter in Italy.]]>67203211Bonardi, S., E. Maggi, G. Pizzin, S. Morabito and A. Caprioli,2001Escherichia coli O157 and carcass contamination in cattle at slaughter in Northern Italy.]]>664753Caprioli, A., S. Morabito, H. Brugere and E. 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