Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.1009.1014Cicer arietinum L.)]]>YousefiaraMahdieh BagheriAbdolreza MoshtaghiNasrin 72008117In this study, multiple shoot induction and whole plant regeneration
from decapitated embryo axes of three chick peal genotypes including MCC252,
MCC283 and MCC505 were evaluated on modified Murashige and Skoog`s medium
(MMS) which, its vitamins were replaced by vitamins of B5 medium, supplemented
with varied concentration of thidiazuron (0.1, 0.2 mg L-1)
or 6-benzylaminopurin (1, 2 mg L-1) or zeatin (1, 2 mg L-1)
treatments. BAP was found to be the most effective cytokinin in normal
multiple shoot induction. Shoots were elongated on growth regulator-free
medium and then rooted on two media containing 1/4 MMS salts and B5 vitamins
+ 3% sucrose + 0.8% agar with indol-3-butyric acid (0.4 or 1 mg L-1).
The highest rooting frequency resulted in a medium including 0.4 mg L-1
IBA. It was found that different shoot induction media also positively
affected rooting, where a medium with 2 mg L-1 BAP in MCC252/MCC505
and a medium with 2 mg L-1 zeatin in MCC283 were the best media
in shoot induction that produced high frequency, thick spread roots. Plantlets
were preliminary acclimatized in liquid medium (1/4 MMS salts and B5 vitamins
+ 3% sucrose + 0.4 mg L-1 IBA) for 7 to 14 days, then transferred
to pots filled by cocopit: perlite (1:1) and kept in a growth chamber
until their shoots and roots were well developed. This resulted in more
than 70% survival rate.]]>Aminuddin, S.K.M. and B.P. Singh,1995Cicer arietinum L.) through in vitro cultures.]]>55963Bajaj, Y.P.S.,19901990Barna, K.S. and A.K. Wakhlu,1993Cicer arietinum L.).]]>12521524Barna, K.S. and A.K. Wakhlu,1994Cicer arietinumfrom callus cultures via organogenesis.]]>13510513Batra, P., N.R. Yadav, A. Sindhu, R.C. Yadav, V.K. Chowdhury and J.B. Chowdhury,2002in vitro direct plant regeneration in chickpea (Cicer arietinum L.).]]>40600602Chaturvedi, C.P. and L. Chand,200182021Eapen, S. and L. George,1994Cicer arietinum L. influence of genotype and auxins.]]>36343349Fernandez-Romero, M.D., M.T. Moreno and J.I. Cubero,1998in vitro rooting method for chickpea.]]>51819Gamborg, O.L., R.A. Miller and K. Ojima,196850151158Huda, S., R. Islam and M.A. Bari,1999Cicer arietinum L.) from callus cultures of internode explants.]]>9107111Huda, S., N.A. Siddique, N. Khatun, M.H. Rahman and M. Morshed,2003Cicer arietinum L.).]]>613101313Ignacimuthu, S. and S. Prakash,200631339345Islam, R. and S. Riazuddin,1993In vitro rooting on micropropagated shoots of chickpea.]]>292123Kiran, G., C.D. Kaviraj, G. Jogeswar, P.B. Kavi Kishor and S. Rao,2005Cicer arietinum L.), a grain legume.]]>8910121018Krishnamurthy, K.V., K. Suhasini, A.P. Sagare, M. Meixner, A. De Kathen, T. Pickardt and O. Schieder,2000Cicer arietinum L.) embryo axes.]]>19235240Kumar, V.D., P.B. Kirti, J.K.S. Sachan and V.L. Chopra,1994Cicer arietinum L.).]]>13468472Kumar, V.D., P.B. Kirti, J.K.S. Sachan and V.L. Chopra,1995Cicer arietinum L.).]]>109207213Malik, K.A. and P.K. Saxena,1992Pisum sativum), chickpea (Cicer arietinum) and lentil (Lens culinaris).]]>19731740Murthy, B.N.S., J. Victor, R.P. Singh, R.A. Fletcher and P.K. Saxena,1996In vitro regeneration of chickpea (Cicer arietinum L.): Stimulation of direct organogenesis and somatic embryogenesis by thidiazuron.]]>19233240Polisetty, P., V. Paul, J.J. Deveshwar, S. Khetarpal, K. Suesh and R. Chandra,1997Cicer arietinum L.).]]>16565571Polowick, P.L., D.S. Baliski and J.D. Mahon,2004Cicer arietinum L.), gene integration, expression and inheritance.]]>23485491Sagare, A.P., K. Suhasini and K.V. Krishnamurthy,1993Cicer arietinum L.).]]>12652655Sanyal, I., A.K. Singh, K. Meetu and D.V. Alma,2005Cicer arietinum L.) with Bacillus thuringiensis Cry1Ac gene for resistance against pod borer insect Helicoverpa armigera.]]>16811351146Senthil, G., B. Williamson, R.D. Dinkins and G. Ramsay,2004Cicer arietinum L.).]]>23297303Shalini, S., P. Batra, A. Sindhu and V.K. Chowdhury,2001Cicer arietinum L.).]]>21308311Sharma, L. and D.V. Alma,1998Cicer arietinum L.).]]>36605609Sheila, V.K., J.P. Moss, C.L.L. Gowda and H.A. Van Rheenen,1991Cicer spp.]]>242830Singh, A., N.P. Singh and A. Asihana,199743940Singh, R., K. Srivastava, H.K. Jaiswal, D.V. Amla and B.D. Singh,200245503508Srivastava, K., K.N. Tiwari, R. Singh, B.D. Singh and H.K. Jaiswal,2001Cicer arietinum.]]>44333337Surya, P., J.B. Chowdhary, P.K. Jain and V.K. Chowdhury,1992Cicer arietinum L.).]]>3011491153