Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.936.940GumusM. SipahiogluH.M. Paylan1I.C. ErkanS. 62007106Although the reverse transcriptase polymerase chain reaction (RT-PCR) procedure is basically simple operation, often it is not possible to achieve optimum results without optimizing the protocols. An RT-PCR method targeting a 200 bp sequence of the CP gene of Apricot Latent Virus (ApLV) was used as a model to improve the detection limit and to compare the behavior of three different plant tissues in a RT-PCR assay. A number of factors should be considered when selecting the optimal system for RT-PCR. Important considerations include the optimal concentrations of MgCl2, dNTP, Taq DNA polymerase enzyme, specific primer and the amount of cDNA for the downstream applications. This study therefore discusses a series of critical PCR parameters and feasible strategies for optimization of RT-PCR detection of ApLV.]]>Adams, M.J., F.J. Antoniw, M. Bar-Joseph, A.A. Brunt and T. Candresse et al.,200414910451060Arezi, B., W. Xing, J.A. Arezi and H.H. Hogrefe,2003321226235Chamberlain, J.S., R.A. Gibbs, J.E. Ranier, P.N. Nguyen and C.T. Caskey,19891989pp: 272-281pp: 272-281Czerny, T.,199624985986Eckert, K.A. and T.A. Kunkel,19901837393744Foissac, X., L. Savalle-Dumas, P. Gentit, M.J. Dulucq and T. Candresse,20003575259Gentit, P., X. Foissac, L. Svanella-Dumas and T. Candresse,2001Apricot latent foveavirus (ALV) isolated from South European orchards associated with peach asteroid spot and peach sooty ringspot diseases.]]>550213219Gumus, M., M. Al Rwahnih and A. Myrta,2004869292Innis, M.A. and D.H. Gelfand,19901990pp: 3-12pp: 3-12Miesfeld, R.,1999pp: 293pp: 293Nemchinov, L. and A. Hadidi,1998472159173Park, K. and J. Lee,20032003Seal, S. and D. Cotes,19981998pp: 469-486pp: 469-486Usta, M., H.M. Sipahioglu and B. Polat,200544189194Yang, D.Y., A. Cannon and S.R. Saunders,200431619631Zemtchik, E.Z. and T.D. Verderevskaya,19933130133Zemtchik, E.Z., T.D. Verderevskaya and Y.A. Kalashian,1998472153158Ghanem-Sabanadzovic, N.A., H. Abbadi, M. Al Rwahnih, M.A. Castellano and A. Myrta,2005873337Sardelli, A.D.,1993915