Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.768.772GharraviA.M. GolalipourM.J. GhorbaniR. KhazaeiM. 52007105Iron weigert staining methods is used as nuclear staining.
In present study we introduce a modification iron weigert hematoxylin
for staining neuron without astrocytes. Whole brain of adult wistar rats
(12-1 3 week old) were removed, immersed in formaldehyde fixative and
embedded in paraffin. Sections, 5-7 μm (from brain cortex, hippocampus,
cerebellum) divided to three groups: one for staining by Hematoxyllin
and eosin, second for staining by cresyl fast vio let (that specially
performed for Nisl substances in neuron) and last for staining by modification
iron hematoxyllin methods, but different in quantity and quality. In new
method general and specific architecture of neuron, nucleus and nuclear
envelope was clearly visible reactions of neuron were predominant. Astrocyte
did not respond to staining methods. Also spines (axon) of purkinje cells
clearly visible. Modification iron weigert hematoxylin can be replacement
to cost and time consuming chemical staining method for staining neurons]]>Gray, P.,1954Horobin, R.W.,1988Kiernan, J.,19993rd Edn.,pp: 31-114pp: 31-114Lillie, R.D., P. Pizzolato, R.A. Welsh, N.D. Holmquist, P.T. Donaldson and C.A. Berger,197360817819Rozas,19351935Luna, L.G.,19603rd Edn.,Weigert, K.E.K.,19041904