Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.506.510ZebaBoukare KiendrebeogoMartin LamienAline DocquierJean-Denis SimporeJacques Germaine NacoulmaOdile 32007103Many clinical species of bacteria were isolated from biological samples
such as urines, blood and wound in Saint Camille medical centre of Ouagadougou.
Among the concerned species, the most important members were Escherichia
coli and Klebsiella pneumoniae. These β-lactamases producing
isolates were directly screened by PCR to identify the nature of the amplified
genes responsible for penicillin destroying activity. Therefore specific
TEM and SHV primers were used. The PCR products were sequenced.
The sequencing results indicated that the parental forms blaTEM-1
and blaSHV-1 were the most common determinants of β-lactamase
found, respectively in Escherichia species and Klebsiella pneumoniae.
The bacterial susceptibility analysis by MICs measurement clearly correlated
the presence of concerned β-lactamase determinants and their resistance
patterns. This study is part of a set of investigations carried out by
our laboratory to assess the β-lactamase incidence in the failure
of β-lactam therapy. In particular, the purpose of this study was
to determine the precise nature of β-lactamase supporting the low
susceptibility of host bacteria towards penicillins.]]>Abraham, E.P. and E. Chain,1940146837837Ambler, R.P.,1975Staphylococcus aureus penicillinase.]]>151197218Ambler, R.P. and G.K. Scott,1978Escherichia coli plasmid R6K.]]>7537323736Ambler, R.P.,1980289321331Barthelemy, M., J. Peduzzi, C. Verchere-Beaur, H.B. Yaghlane and R. Labia,19861371927Bush, K.,19881109123Bush, K., G.A. Jacoby and A.A. Medeiros,19953912111233Dubois, V., L. Poirel Carping, L. Coulange, C. Bebear, P. Nordmann and C. Quentin,2004Klebsiella pneumonie.]]>4844664469Essack, S.Y., L.M.C. Hall, D.G. Pillay, M.L. McFadyen and D. Livermore,2001Klebsiella pneumoniae Strains with Extended-Spectrum β-lactamases isolated in 1994 and 1996 at a Teaching Hospital in Durban, South Africa.]]>458895Fleming, A.,1929Penicillium, with special reference to their use in the isolation of B. influenzae.]]>10226236Gangoue-Pieboji, J., B. Bedenic, S. Koula-Shiro, C. Randegger and D. Adiopo et al.,20054332733277Hujer, A.M., K.M. Hujer and R.A. Bonomo,200115473750Majiduddin, F.K. and T. Palzkill,2003163456466Maveyraud, L., I. Saves, O. Burlett-Schiltz, P. Swaren and J.M. Masson et al.,19962711048210489Medeiros, A.A.,1984401827Pitout, J.D.D., K.S. Thompson, N.D. Hanson, A.F. Ehrhardt, E.S. Moland and C.C. Sander,1998Klebsiella pneumoniae, Escherichia coli and Proteus mirabilis isolates recovered in South Africa.]]>4213501354Zeba, B., J. Simpore, O. Nacoulma and J-M. Frere,20054286288Du Bois, S.K., M.S. Marriott and S.G. Amyes,197535722