Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.481.485Medicago sativa and Medicago truncatula ]]>HooriF. EhsanpourA.A. MostajeranA. 32007103In this study, the regeneration through embryogenesis of two species
of Medicago were studied. Seeds of Medicago sativa cv. Rehnani
and M. truncatula line A17 were grown on MS medium. After 4-6 weeks,
segments of leaf and stem from two species were transferred to MS medium
containing 2 mg L-1 NAA, 2,4-D and Kinetin. The results indicated
that callus formation from leaf explants of M. sativa was higher
than M. trancatula. In the next stage, media with different combinations
of auxin, cytokinin or ethinyl estradiol were provided for regeneration.
Then in two stages, explants of leaf and stem of two species were transferred
on these media. Results after 3-6 weeks showed that in medium containing
NAA and TDZ, stem pieces of M. sativa produced shoots while leaf
pieces on NAA and ethinyl estradiol formed roots. Leaf explants of
M. truncatula in the medium containing NAA and BAP, produced somatic
embryos. Also in media with auxin and ethinyl estradiol, somatic embryos
were formed on calli of two species. Ethinyl estradiol and auxin together
can induce somatic embryogenesis and root production on calli and stem
or leaf explants.]]>Bajguz, A. and A. Tretyn, 20036210271046Araujo, S., A. Duque, D. Santos and M. Fevereiro,2004Medicago truncatula cv. Jemalog.]]>78123131Bao, F., J. Shen, S. Brady, G. Muday and T. Asami,2004Arabidopsis.]]>13416241631Barker, D.J., S. Bianchi, F. Blondon, Y. Dattee and G. Duc et al.,1990Medicago truncatula, a model plant for studying the molecular genetics of the Rhizobium-legume symbiosis.]]>84049Crawford, E.J., A.W.H. Lake and K.G. Boyce,1989Medicago species for semiarid conditions in Australia.]]>42399435Dudtis, D., L. Bogre and J. Guorgyey,1991in vitro.]]>99475484Goda, H., S. Sawa, T. Asami, S. Fujioka, Y. Shimada and S. Yoshida,2004Arabidopsis.]]>13415551573Huetteman, C.A. and J.E. Preece,199333105119Iantcheva, A., M. Vlahova, E. Balakova, E. Kondorosi, M.C. Elliott and A. Atanassov,199918904910Matand, K., D. Porodo and C.S. Prakash,1994Arachys hypogaea) plants in vitro.]]>1415McKersie, B.D. and D.C.W. Brown,1997pp: 111-132pp: 111-132Murashige, T. and F. Skoog,196215473497Neves, L.O., S.R.L. Duque, J.S. Almeida and P.S. Fevereiro,1999Medicago truncatula sp. Narbonensis and Medicago truncatula Gaertn. cv. Jemalong.]]>18398405Nolan, K.E., R.J. Rose and J.R. Gorst,1989Medicago truncatula from tissue culture: Increased somatic embryogenesis using explants from regenerated plants.]]>8278281Nolan, K.E. and R.J. Rose,1998Medicago truncatula with particular reference to abscisic acid and light treatments.]]>46151160Nolan, K.E., R.R. Irwanto and R.J. Rose,2003Medicago truncatula root forming and embryogenic cultures.]]>133218230Rawn, J.,1989pp: 557-570pp: 557-570Rose, R.J. and K.E. Nolan,1995Medicago truncatula from protoplasts isolated from kanamycin sensitive and kanamycin-resistant plants.]]>14349353Von Arnold, S., I. Sabala, P. Bozhkov, J. Dyachok and L. Filonova,200269233249Saunders, J.W. and E.T. Bingham,197212804808Somers, D.A., D.A. Samac and P.M. Olhoft,2003131892899