Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.4449.4454BhowmikSalil KumarIslamMirza MofazzalEmonReza MohammadBegumShamsun NaharSiddikaAysha SultanaSharmin 1220071024Eleven genotypes, including the salt tolerant cultivar
Pokkali as check, were used to evaluate salinity tolerance phenotypically
and genotypically. Three selected SSR primers viz., RM7075, RM336 and
RM253 were used to evaluate rice genotypes for salt tolerance. Two setups
were maintained for this study viz., the seedling and reproductive stages.
Phenotyping at the seedling stage was done in hydroponic system using
salinized (EC 12 dS m-1) nutrient solution and at the reproductive
stage using salinized tap water (EC 6 dS m-1). IRRI standard
protocol was followed to evaluate salinity tolerance in rice.The
genotypes having similar banding pattern with Pokkali were considered
as tolerant. Phenotypically, three genotypes Pokkali, THDB and TNDB-100
and five genotypes RD-2586, TNDB-100, Dhol Kochuri, PNR-519 and Pokkali
were identified as salt tolerant at the seedling and reproductive stages,
respectively. These genotypes were also identified as salt tolerant genotypically
(with markers). Through phenotypic and genotypic study, five genotypes
viz., Pokkali, Dhol Kochuri, RD-2586, TNDB-100 and PNR-519 were identified
as salt tolerant. Therefore, these microsatellite markers used in this
study could be efficiently used for identification of salt tolerant rice
varieties in marker-assisted breeding and quantitative trait loci analysis.]]>Baloch, A.W., A.M. Soomro, M.A. Javed, H.R. Bughio and S.M. Alam et al.,20032273276Bhuiyan, M.A.R.,20052005Bonilla, P.S., J. Dvorak, D. Mackill, K. Deal and G. Gregorio,2002Oryza saliva L.) using recombinant inbred lines.]]>856476Evers, D., C. Schmit, Y. Mailliet and J.F. Hausman,1997in vitro under sodium chloride stress.]]>151748753Foolad, M.R.,1999165564Gregorio, G.B. and D. Senadhira,1993Oryza sativa L.).]]>86333338Gregorio, G.B.,19971997Gregorio, G.B., D. Senadhira and R.D. Mendoza,19971997Gregorio, G.B., D.R.D. Senadhira, R.D. Mendoza, N.L. Manigbas, J.P. Roxas and C.Q. Guetra,20027691101Islam, M.M.,2004Oryza sativa L.) at reproductive stage.]]>2004Lang, N.T., S. Yanagihara and B.C. Buu,200083748Lopez, M.V. and S.M.E. Satti,19961141927Manneh, B.,2004Oryza sativa L.).]]>2004Mishra, B., M. Akbar and D.V. Sashu,19901990pp: 25Misra, M., N. Das and A.N. Misra,1995Pennisetum glaucum L. R. br.).]]>17371374Mori, I.K. and T. Kinoshita,19874112113Delane, R.H., H. Greenway, R. Munns and J. Gibbs,1982Hordeum vulgar growing at high external NaCl.]]>33557563Nguyen, T.L., L. Zhikang and B.C. Buu,20019921Niones, J.M.,2004Oryza sativa L.) using near-isogenic lines.]]>2004Peng, S., K.G. Cassman, S.S. Vimani, J. Sheehy and G.S. Khush,19993915521559Qian, Y.L., S.J. Wilhelm and K.B. Marcum,20014118951900Salim, M., R.C. Saxena and M. Akbar,199030654659Smith, S. and T. Helentjaris,19961996pp: 95-110pp: 95-110Suplick-Ploense, M.R., Y.L. Qian and J.C. Read,20024220252030Xiao, J., J. Li, S.R. McCouch and S.D. Tanksley,199692637643Yoshida, S., D.A. Forno, J.H. Cock and K.A. Gomez,1976pp: 61-66pp: 61-66Zeng, L. and M.C. Shannon,2000409961003Zhang, Z.H., P. Li, L.X. Wang, Z.L. Hu, L.H. Zhu and Y.G. Zhu,200416718