Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.3708.3712Gomphrena globosa L.]]>GhaffarIram AliBasharat HasnainShahida 1220071020The main aim of this study was to observe the effect of different hormonal combinations on regeneration of callus of Gomphrena globosa L. For this purpose callus was obtained from seeds G. globosa inoculated on MS medium supplemented with 4 mg L-1 2, 4-D and 10% coconut milk. After callus formation callus was inoculated on Murashige and Skoogs medium supplemented with different combinations of BAP, NAA and GA3 to observe different responses such as regeneration, callus friability, callus proliferation and pigmentation. In BAP and NAA root regeneration was observed at 1.5 mg L-1 BAP+1 mg L-1 NAA whereas rest of the combinations showed callus proliferation. In BAP and GA3, root regeneration was observed in most of the combinations and some combinations also showed shoot induction. Shoot regeneration was observed on 0.5 mg L-1 BAP + 7 mg L-1 GA3 and 1 mg L-1 BAP + 0.2 mg L-1 GA3. The effect of all these combinations on auxin, acid phosphatase and soluble protein content was also observed.]]>Garcia-Martin, G., J.A. Manzanera and M.E. Gonzalez-Benito,2005Quercus suber somatic embryos.]]>80171177Iqbal, J. and N. Rafique,19862 on germination, early seedling growth, soluble proteins and acid phosphatase in Zea mays L.]]>1918Emma, M.J., A. Sevdalina and A.C.J. Verkleij,2005Silene vulgaris (Caryophyllaceae).]]>802531Jimenez, V.M. and F. Bangerth,2001673746Lans, C.A.,20062006Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Mahadevan, A.,1984pp: 31pp: 31Mercier, H., C.C.J. Vieira and R.C.L. Figueiredo-Ribeiro,1992Gomphrena offcinalis-a Brazilian medicinal plant.]]>28249254Moura, R.M.X., P.S. Pereira, A.H. Januario, S.D. Franca and D.A. Dias,2004Gomphrena celosioides by TLC-Densitometry.]]>5213421344Murashige, T. and F. Skoog,196215473497Ruiz, A.Q. and J.M. Valadez,1985Gomphrena globosa callus tissue.]]>48976980Vieira, C.C.J., H. Mercier, E.P. Chu and R.C.L. Figueiredo-Ribeiro,1994pp: 257-270pp: 257-270Vieira, C.C.J., M.R. Braga and R.C.L. Figueiredo-Ribeiro,1995Gomphrena macrocephala St.-Hil.]]>42233238