Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.3079.3084strR, an Antibiotic Regulatory Gene, using RFLP and Nested PCR]]>HojatiZohreh Motovali-BashiMajid GolbangNaser DarvishiFarshad 1220071018The genetics of streptomycin production is well characterized in Streptomyces griseus. More than 25 clustered genes encode proteins involved in biosynthesis, regulation and transport functions. StrR, the pathway specific transcriptional activator or regulator that located in this cluster, then induces transcription of other streptomycin production genes by binding multiple sites in the gene cluster. We aim to put strR gene in to different multicopy and integrated expression vector specifically designed for Streptomyces. To start with, the isolated strR gene was ligated into pBluescript (pBs) vector and transformed into different strains of Escherichia coli. The correct structure of the recombinant plasmid, isolated from transformed E. coli, was confirmed using gel electrophoresis, PCR and double digested with restriction enzymes BamHI and EcoRI. Finally the plasmid map, named pFDstrR. This unique vector has a much expanded Multiple Cloning Site (MCS), which makes it suitable for different purposes of gene cloning and also site directed mutagenesis or gene targeting. This gene will be lifted up and transfer into different varieties of Streptomyces specific vectors in order to make different transgenic or genetically manipulated Streptomyces.]]>Aigle, B., A. Wietzorrek, E. Takano and M.J. Bibb,2005Streptomyces antibiotic regulatory protein family, in regulation of the duplicated type II polyketide synthase alp gene cluster in Streptomyces ambofaciens.]]>18724912500Anderson, A.S., D.J. Clark, P.H. Gibbons and J.M. Sigmund,2002296069Babcock, M.J. and K.E. Kendrick,1988Streptomyces griseus.]]>17028022808Browning, D.F. and S.J. Busby,200425765Darvishi, F.,2005Escherichia coli strains using pFDstrR.]]>2005Darvishi, F., N. Gholbangh, Z. Hojati and M. Motovali-Bashi,2006strR) by PCR.]]>19259272Egan, S., P. Wiener, D. Kallifidas and E.M.H. Wellington,1998Streptomycetes isolated from soil.]]>6450615063Hesketh, A., J. Sun and M. Bibb,2001Streptomyces coelicolor A3(2) grown under conditions of nutritional sufficiency elicits actII-ORF4 transcription and actinorhodin biosynthesis.]]>39136144Hojati, Z.,2002Streptomyces coelicolorA3(2).]]>2002Hojati, Z., C. Milne, B. Harvey, L. Gordon and M. Borg et al.,2002Streptomyces coelicolor.]]>911751187Hong, B., S. Phornphisutthimas, E. Tilley, S. Baumberg and J.K. McDowall,2007Streptomyces griseus can be modulated by a mechanism not associated with change in the adpA component of the A-factor cascade.]]>295764Horinouchi, S., Y. Ohnishi and D.K. Kang,2001Streptomyces griseus.]]>27177182Kendrick, K.E. and J.C. Ensign,1983Streptomyces griseus in submerged culture.]]>155357366Kieser, T., M.J. Bibb, M.G. Buttner, K.F. Chatter and D.A. Hopwood,2000Streptomyces Genetics.]]>2nd Edn.,Kim, D.W., K.F. Chater, K.J. Lee and A. Hesketh,2005Streptomyces coelicolor.]]>15127072720Ohnishi, Y., S. Kameyama, H. Onaka and S. Horinouchi,1999Streptomyces griseus: Identification of a target gene of the A-factor receptor.]]>34102111Ohnishi, Y., H. Yamazaki, J.Y. Kato, A. Tomono and S. Horinouchi,2005Streptomyces griseus.]]>69431439Ramón-García, S. I. Otal, C. Martín, R. Gómez-Lus and J.A. Aínsa,2006Mycobacterium fortuitum.]]>5039203922Retzlaff, L. and J. Distler,1995strR, of Streptomyces griseus is a DNA binding activator protein with multiple recognition sites.]]>18151162Schatz, A. and S.A. Waksman,1944Mycobacterium tuberculosis and related organisms.]]>57244248Vujaklija, D., S. Horinouchi and T. Beppu,1993Streptomyces griseus.]]>17526522661Yamazaki, H., Y. Ohnishi and S. Horinouchi,2000adsA) that is essential for morphological development in Streptomyces griseus.]]>18245964605Yamazaki, H., Y. Ohnishi and S. Horinouchi,2003Streptomyces griseus.]]>18512731283Yamazaki, H., Y. Takano, Y. Ohnishi and S. Horinouchi,2003Streptomyces griseus.]]>5011731187