Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.2639.2645SrisamootNattapong ChaveerachArunrat NuchadomrongSuporn SattayasaiNison ChaveerachPrapansak TanomtongAlongkoad PinthongKrit 1220071016The cytogenetics of eight Felidae species in Thailand were investigated by the colchicines-hypotonic fixation-air drying technique followed by a conventional technique. All species studied have an identical number of 38 diploid chromosomes, indicating a close genetic relationship among species. At a deep study level, the genetic relationships of eight Felidae species were accessed by the AFLP method. Blood samples were collected from sources locating in their original regions for DNA extraction. With ten successful primer combinations, a total of 4208 scorable bands were generated. Of these bands, 18.91% are polymorphic. Percentages of Polymorphic Bands (PPB) for each primer combination range from 15.00 to 23.59%. The generating bands were used for dendrogram construction. The average genetic similarity values among all Felidae species are 68.20% (between Panthera tigris and Neofelis nebulosa) to 85.53% (between Prionailurus bengalensis and Prionailurus viverrinus). The dendrogram shows that the eight Felidae species were clustered together and the subfamily Pantherinae and Felinae with Neofelis nebulosa are distinguished. The Felinae, Prionailurus bengalensis, Prionailurus viverrinus, Catopuma temminckii, Felis chaus, Pardofelis marmorata and Neofelis nebulosa were clustered together with 91% bootstrap support and the Pantherinae, Panthera pardus is clustered with Panthera tigris with 92% bootstrap support. In summary, the ten successful primer combinations can be used to determine genetic differences among eight Thailand Felidae species.]]>Baillie, J.E.M., C. Hilton-Taylor and S.N. Stuart,2004Threatened Species.]]>pp: 191pp: 191Benveniste, R.E. and G.J. Todaro,19747033163320Benveniste, R.E., C.J. Sherr and G.J. Todaro,1975190886888Bininda-Emonds, O.R.P., J.L. Gittleman and A. Purvis,199974143175Bininda-Emonds, O.R.P., D.M. Decker-Flum and J.L. Gittleman,200172115Collier, G.E. and S.J. O`Brien,198539473487Flynn, J.J., J.A. Finarelli, S. Zehr, J. Hsu and M.A. Nedbal,200554317337Gupta, P.K., R.K. Varshney, P.C. Sharma and B. Ramesh,1999118369390Hsu, T.C. and H.H. Rearden,196516365371Janczewski, D.N., W.S. Modi, J.C. Stephens and S.J. O`Brien,199512690707Johnson, W.E., P.A. Dratch, J.S. Martenson and S.J. O`Brien,1996397120Johnson, W.E. and S.J. O’Brien,199744S98S106Johnson, W.E., J.A. Godoy, F. Palomares, M. Delibes, M. Fernandes, E. Revilla and S.J. O’Brien,2004951928Koepfli, K., S.M. Jenks, E. Eizirik, T. Zahirpour, B.V. Valkenburgh and R.K. Wayne,200638603620Lopez, J.V., N. Yuhki, R. Masuda, W. Modi and S.J. O'Brien,199439174190Mattern, Y.M. and A.D. McLennan,200016232253Modi, W.S. and S.J. O’Brien,19881988Neff, N.A.,1982O'Brien, S.J., G.E. Collier, R.E. Benveniste, W.G. Nash and A.K. Newman et al.,19871987Ortolani, A.,199967433476Pathak, S. and D.H. Wurster-Hill,197718245254Pecon-Slattery, J., W.E. Johnson, D. Goldman and S.J. O’Brien,199439296305Pecon-Slattery, J., A.J. Pearks Wilkerson, W.J. Murphy and S.J. O’Brien,20042122992309Pejic, I., P. Ajmone-Marsan, M. Morgante, V. Kozumplick, P. Castiglioni, G. Taramino and M. Motto,19989712481255Peters, G. and M.H. Hast,19945987104Postanowicz, R.,20062006Powell, W., M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingey and A. Rafalski,19962225238Reeves, R.H. and S.J. O`Brien,198452164171Russell, J.R., J.D. Fuller, M. Macaulay, B.G. Hatz, A. Jahoor, W. Powell and R. Waugh,199795714722Salles, L.O.,19923047167Sambrook, J., E.F. Fritsch and T.A. Maniatis,19892nd Edn.,Pages: 397Pages: 397Sato, J.J., M. Wolsan, H. Sizuki, T.Hosoda, Y. Yamaguchi, K. Hiyama, M. Kobayashi and S. Minami,200623125146Tanomtong, A., S. Khunsook, W. Kaensa and R. Banjongrat,2005Macaca nemestrina) by conventional staining.]]>13111Tian, Y., W. Nie, J. Wang, M.A. Ferguson-Smith and F. Yang,2004125563Uphyrkina, O., D. Miquelle, H. Quigley, C. Driscoll and S.J. O’Brien,2002Panthera pardus orientalis).]]>93303311Vos, P., R. Hogers, M. Bleeker, M. Reijans and T. van de Lee et al.,19952344074414Wilson, D.E. and D.M. Reeder,20063rd Edn.,Wurster-Hill, D.H. and K. Benirschke,196824336382Wurster, D.H.,19691969Wurster-Hill, D.H. and C.W. Gray,197312377379Wurster-Hill, D.H. and W.R. Centerwall,198234178192Zhang, W., Z. Zhang, F. Shen, R. Hou, X. Lv and B. Yue,2006Panthera tigris).]]>85107116