Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2007.2056.2060 Plant Regeneration Through Anther Culture of Some Iranian Local Rice (Oryza sativa L.) Cultivars]]>TalebiReza RahemiMohammad RezaArefiHabibolah NouroziMohammad BagheriNadali 1220071012In this study, effect of different genotypes, different callus induction and regeneration media were investigated using seven Iranian local rice. Anthers were cultured on N6, Fj and L8 media containing the same hormonal combination 3 mg L-1 NAA, 0.5 mg L-1 Kn and 0.5 mg L-1 2, 4-D and incubated at 25±
1°
C in dark for callus induction. All varieties in L8 medium, five varieties in Fj medium and six varieties in N6 medium produced highest percentage of callus. Calli induced in different induction media were transferred to SK 11 and N 19 medium and incubated at 25±
1°
C in light for plantlet regeneration. Among seven varieties upon transfer to SK 11 medium, highest percentage (40%) of green plants were produced in Hassani and in N 19 medium the highest percentage (15.78) of green plants and albino plants (21.05) were produced in Anbarbo. The finding in the present investigation showed that the successfully emberyogenesis and green plant regeneration in rice anther culture dependent on medium culture components and are affected by the genetic mack-up of the plants.]]>Asaduzzaman, M., M.A. Bari, M.H. Rahman, N. Khatun, M.A. Islam and M. Rahman,2003In vitro plant regeneration through anther culture of five rice varieties.]]>3167171Bhaskaran, S. and R.H. Smith,19903013281337Bhojwani, S.S. and M.K. Razdan,1996A Revised Edn.Cao, M.Q., F. Li, T. Jiang and G.S. Li,19953922738Chen, C. and M. Lin,1976171824Chu, C.C.,19821982pp: 129-158pp: 129-158Chu, C.C., C.C. Wang, C.S. Sun, K.C. Hsu, K.C. Yin, C.Y. Chu and F.Y. Bi,197518659668Dunwell, J.M.,19861986pp: 375-404pp: 375-404Guha-Mukejee, S.,1973in vitro formation and embryoids from rice pollen.]]>24139144Hu, H.,198511123IRRI,1992Kao, K.N., M. Saleem, S. Abrams, M. Pedras, D. Horn and C. Mallar,19919595601Lentini, Z., P. Reyes, C.P. Martinez and W.M. Roz,1995110127138Miah, M.A.A., E.D. Earle and G.S. Kush,1985Oryza sativa L.]]>70113116Murashige, T. and F. Skoog,196215473497Karim, N.H.N., A.K.M. Shahjahan, Maksuda, A. Nahar, S.A. Miah and M.Z. Haque,1991Oryza sativa).]]>14350Ogawa, T., H. Fukuwa and Y. Okhawa,199545301307Oono, K.,1975Oryza sativa) by anther culture and their use for breeding.]]>26139222Raina, S.K. and F.J. Zapata,1997Oryza sativa L.) through modification of the culture media.]]>116305315Roy, B. and A.B. Mandal,20054235240Villenx, R.E.,19942912381240