Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2006.2887.2891In vitro Anti-Helicobacter pylori Effects of Sweet Basil (Ocimum basilicum L.) and Purple Basil (Ocimum basilicum var. purpurascens)]]>NakhaeiM. MahboobehMalekzadehFeridounKhaje-KaramoddinMehrangizRamezaniMohammad122006915Anti-Helicobacter pylori effects of leaves of Ocimum basilicum L. (sweet basil, Lamiaceae) and Ocimum basilicum var. purpurascens (purple basil, Lamiaceae) were evaluated. Macerated aqueous and methanol extracts were tested against 45 clinical isolates of Helicobacter pylori using paper Disc Diffusion Method (DDM) on modified egg yolk emulsion agar (EYE agar). Although there were small differences in sensitivity among the isolates tested, but all isolates were susceptible to methanol and aqueous extracts. The Minimum Inhibitory Concentrations (MIC) of the extracts from leaves of sweet basil and purple basil were 677 and 729 μg mL-1, respectively using agar dilution method. Antibacterial constituents of sweet basil extract were stable after one month of storage at 4°C but lost 31% its activity after 4 weeks at room temperature. The extract preserved its activity when heated to 80°C for 30 min and autoclaved at 121°C for 20 min. It was stable at pH 5-8.]]>Boyanova, L., R. Koumanova, E. Lazarova and C. Jelev, 2003Helicobacter pylori and Helicobacter heilmannii in children. A Bulgarian study.]]>Diag. Microbiol. Infect. Dis.,46249252Brain, K.R. and T.D. Turner,1975The Practical Evaluation of Phytopharmaceuticals.1st Edn.,pp: 10-30pp: 10-30Cellini, L., E.D. Campli, M. Masulli, S.D. Bartolomeo and N. Allocati,1996Helicobacter pylori by garlic extract (Allium sativum).]]>FEMS Immunol. Med. Microbiol.,13277279Chhabra, S.C., F.C. Uiso and E.N. Mshiu,1984J. Ethnopharmacol.,11157179Edwards, D.I.,1993J. Antimicrob. Chemother.,31920Goodwin, C.S. and J.A. Armstrong, 1990Helicobacter pylori.]]>Eur. J. Clin. Microbiol. Infect. Dis.,9113Goddard, A.F. and R.P.H. Logan, 1996Helicobacter pylori.]]>J. Antimicrobiol. Chemother.,37639643Javanmardi, J., A. Khlighi, A. Kashi, H.P. Bais and J.M. Vivanco,2002O. basilicum L.) found in local accessions and used in traditional medicine in Iran.]]>J. Agric. Food Chem.,5058785883McNulty, C., R. Owen, D. Tompkins, P. Hawtin, K. McColl, A. Price, G. Smith and L. Teare, 2002Helicobacter pylori susceptibility testing by disc diffusion.]]>J. Antimicrob. Chemother.,49601609Opalchenova, G. and D. Obreshkova,2003Ocimum basilicum L. against multi-drug resistant clinical isolates of the genera Staphylococcus, Enterococcus and Pseudomonas by using different test methods.]]>J. Microbiol. Methods,54105110Oxenham, S.K.,2003Classification of an ocimum basilicum germplasm collection and examination of the antifungal effects of the essentialk oil of basil.2003Oxenham, S.K., K.P. Svoboda and D.R. Walters,2005Ocimum basilicum).]]>J. Phytopathol.,153174180Perry, L.M.,1980Medicinal Plants of East and Southeast Asia: Attributed Properties and Uses.1st Edn.,Pages: 632Pages: 632Shahamat, M., U.E.H. Mai, C. Paszko-Kolva, H. Yamamoto and R.R. Colwell,1991Helicobacter pylori.]]>J. Clin. Microbiol.,2928352837Sinha, G.K., and B.C. Gulati,1990Ind. Perfumer.,34126129Stamatis, G., P. Kyriazopoulos, S. Golegou, A. Basayiannis, S. Skaltsas and H. Skaltsas,2003In vitro anti-Helicobacter pylori activity of Greek herbal medicines.]]>J. Ethnopharmacol.,88175179Tabak, M., R. Armon and I. Neeman,1999Helicobacter pylori.]]>J. Ethnopharmacol.,67269277Ten Brink, B., M. Minekus, J.M.B.M. Vander Vossen, R.J. Leer and J.H.J. Huis,1994J. Applied Bacteriol.,77140148Tytgat, G.N. and E.A. Rauws,1989Campylobacter pylori in gastroduodenal diseases. A believer's point of view.]]>Gastroenterol. Clin. Biol.,13118B121BWorrel, J.A. and S.C. Stoner,1998Helicobacter pylori.]]>Med. Update Psychiatrsits,499104Zargari, A.,1997Medicinal Plants.pp: 403-404pp: 403-404