Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2006.191.196 Escherichia coli from Diarrheic Calves and Lambs by Multiplex Polymerase Chain Reaction]]>SalehiT. ZahraeiSafarchiA. KhorasganiM. Rabbani2200692The purpose of this study was to determine the presence of selected virulence genes in Escherichia coli isolated from diarrheic calves (Tehran province) and lambs (Ghume provinces) of Iran. We examined 40 isolates (29 from calves and 11 from lambs). All 40 isolates were tested for the presence of stx1, stx2, eae, espB and hly genes by multiplex polymerase chain reaction in two protocols. In the first protocol the isolates were tested with EC and hly primers and in the second protocol the isolates were examined with eae, stx1, stx2 and espB primers. Multiplex PCR showed that from 29 strains isolated from diarrheic calves, 4 of isolates (13.7%) were stx1 positive, 16 isolates (55.17%) carried stx2 and 2 isolates (6.89%) had espB gene. 1 isolate (3.44%) possessed eae. Among 11 strains isolated from diarrheic sheep, 9 isolates (81.81%) carried stx2 and 2 isolates (18.18%) had espB gene. Six isolates (54.54%) possessed eae and none of them was stx1 positive. The hly gene was not detected in any of the isolates. The findings of this study indicated that the stx2 may be widespread among pathogenic Escherichia coli in Iran.]]>Agin, T.S. and M.K. Wolf,199765320326Anjum, M.F., S. Lucchini, A. Thompson, J.C.D. Hinton and M.J. Woodward,2003Escherichia coli pathogens.]]>7146744683Blanco, J., D. Cid, J.E. Blanco, M. Blanco, R. Santa, J.A. Quiteira and R. De La Fuente,1996Escherichia coli strains isolated from diarrheic lambs in Spain.]]>5414461451Blanco, M., J.E. Blanco, J. Blanco, A. Mora and C. Prado et al.,1997Escherichia coli (VTEC) isolated from healthy cattle.]]>54309319Armstrong, G.L., J. Hollingsworth and J.G. Morris,1996Escherichia coli O157: H7 as a model of entry of a new pathogen into the food supply of the developed world.]]>182951Call, D.R., F.J. Brockman and P.D. Chandler,2001Escherichia coli O157:H7 using multiplex PCR and nucleic acid microarray.]]>677180China, B., E. Jacwuemin, V. Pirson and A. Devrin,1999150323332Cid, D., J.A. Ruiz-Santa-Quiteria, I. Marin, R. Sanz, J.A. Orden, R. Amils and R. De La Fuente,2001eae) and espB gene subtypes in attaching and effacing Escherichia coli strains isolated from diarrhoeic lambs and goat kids.]]>14323412353Donnenberg, M.S., J. Yu and J.B. Kaper,199317546704680Emery, D.A., K.V. Nagaraja, D.P. Shaw, J.A. Newman and D.G. White,1992Escherichia coli associated with colisepticemia in chickens and turkeys.]]>36504511Fantinatti, F., W.D. Silveria and A.F.P. Castro,1994Escherichia coli strains.]]>417586Frankel, G., A.D. Philips, I. Rossenshine, G. Dougan, J.B. Kaper and S. Knuttoh,1998Escherichia coli: More subversive elements.]]>30911921Foubister, V., I. Rosenshine, M.S. Donnenberg and B.B. Finlay,1994Escherichia coli is necessary for signal transduction in epithelial cells.]]>6230383040Hallaran, G. and J. Sumner,200179707708Jerse, A.E., J. Yu, B.D. Tall and J.B. Kaper,1990Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells.]]>8778397843Khan, A., S.C. Das, T. Ramamurthy, A. Sikdar, J. Khanam, S. Yamasaki, Y. Takeda and G.B. Nair,2002Escherichia coli isolated from divers source in Calcutta, India.]]>4020092015McDaniel, T.K., K.G. Jarvis, M.S. Donnenberg and J.B. Kaper,19959216641668McDaniel, T.K. and J.B. Kaper,1997Escherichia coli confers the attaching and effacing phenotypes on E. coli K-12.]]>23399407Monserrat, S.A., R. Heinz, F.J. Conraths and G. Lutz,2003Escherichia coli in cattle herds.]]>4157605763Nataro, J.P. and J.B. Kaper,1998Escherichia coli.]]>1142201O'Brien, A.D. and R.K. Holmes,198751206220Osek, G.,2003Escherichia coli strains, their major virulence factors gene.]]>951217Osek, J. and P. Gallien,2002Escherichia coli O157 strains isolated from cattle and pigs by the use of PCR and plused-fiold gel electrophoresis methods.]]>43149158Orden, J.A., M. Yuste, D. Cid, T. Piacesi, S. Martinez, J.A. Ruiz-Santa-Quiteria and R. De La Fuente,2003Escherichia coli isolates from ruminants.]]>96203215Parriera, V.R. and G.C. Gyles,2002Escherichia coli.]]>87341352Paton, J.C. and A.W. Paton,1998Escherichia coli infections.]]>11450479Rey, J., J.E. Blanco, M. Blanco, A. Mora and G. Dahbi et al.,2003Escherichia coli isolated from sheep in Spain.]]>944756Yamamoto, S., A. Terai, K. Yuri, H. Kurazono, Y. Takeda and O. Yoshida,1995Escherichia coli by multiplex PCR.]]>128590Yokoigawaka, I., K. Inoue, Y. Okubo and H. Kawai,1999Escherichia coli strains in food.]]>64571575Zschock, M., H.P. Hamann, B. Kloppert and W. Wolter,2000Escherichia coli in faeces of healthy dairy cows, sheep and goats: Prevalence and virulence properties.]]>31203208