Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2005.679.682Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) and their Effects on Acetylcholinesterase Activity]]>Al-RajhyDiefAlla H.HusseinHamdy I.Al-ShawafAbdul-Monem A.5200585The insecticidal activity of carbaryl alone and its mixture with
Piperonylbutoxide (PBO) was tested against males, females and larvae of the Red
Palm Weevil (RPW), Rhynchophorus ferrugineus (Olivier), by both the feeding
and the topical application methods. In addition, the Acetylcholinesterase
(AChE) activity, the in vitro effect of carbaryl alone and the in vivo
effects of carbaryl, PBO and their mixture on the AChE activity were estimated
in RPW. A modification was carried out to enable estimation of AChE activity in
this destructive pest. Tested materials were more toxic by feeding than by topical
application and larvae were more susceptible than adults. In case of food treatment,
the LC50 values of carbaryl alone were 1.183, 1.43 and 0.0367% for
males, females and larvae, respectively. PBO had great synergistic effect on carbaryl
toxicity, the LC50 values for carbaryl+PBO were 0.0155, 0.0253 and
0.00556%against males, females and larvae, respectively. The topical
LD50 values for carbaryl and carbaryl+PBO against larvae were 27.1
and 14.4 μg/larva, respectively. The inhibition percentages of AChE activity in
larvae fed on carbaryl and (carbaryl+PBO)-treated sugarcane were 0.8 and 64.4%,
respectively. The results indicated the great role of oxidases in the reduction
of carbaryl toxicity and their inhibition by PBO.]]>Cox, M.L.,1993413031Abraham, V.A., M.A. Al-Shuaibi, J.R. Faleiro, R.A. Abuzuhairah and P.S.P.V. Vidyasagar,1998Rhynchophorus ferrugineus Oliv, a key pest of date palm in Middle East.]]>37784Murphy, S.T. and B.R. Briscoe,19992035N46NKurian, C. and K. Mathen,1971212931Mathen, K. and K.C. Kurian,1970178Subba-Rao, P.V., T.R. Subramaniam and E.V. Abraham,197312627Abraham, V.A., K.M.A. Koya and C. Kurian,1975Rhynchophorus ferrugineus F.]]>37172Ganeswara, R.A., K. Laxminarayana and R.P. Ramamohana,1980Rhynchophorus ferrugineus Fab in coconut.]]>1156Sathiamma, B., V.A. Abraham and C. Kurian,1982122729Rao, A.G., R.P. Ramamohana, R.T. Ramamohana and K. Lakshminarayana,1989Rhynchophorus ferrugineus Fab in coconut.]]>191216Rajan, P. and C.P.R. Nair,19972724Ajlan, A.M., M.S. Shawir, M.M. Abo-el-Saad, M.A. Rezk and K.S. Abdulsalam,2000Rhynchophorus ferrugineus (Olivier).]]>11526El-Ezaby, F.,1997in vitro study on the red indian date palm weevil.]]>158487Finney, D.J.,19713rd Edn.,pp: 318pp: 318Ellman, G.L., K.D. Courtney, V. Andres Jr. and R.M. Featherstone,196178890Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Solomon, S.S., L.I. Shao-Nan and F. de Fan,2000In vitro inhibition and recovery of brain acetylcholinesterase in Topmouth Gudgeon (Pseudorasobora parva) following exposure to fenitrothion.]]>1448458