Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2005.278.280Staphylococcus Aureus Strains Isolated
from Human, Animals and Environment]]>WisalM. E.HamidM. A.HadiaE. A.JaliiI.M. ElAliA. S.2200582A total of 29 Staphylococcus aureus strains isolated from human, animals and their environment were subjected to genotypic analysis on the basis of coagulase gene polymorphism. The coagulase gene was amplified using a pair of oligonucleotide nested primers. Presumptive phenotypic identification of the strains showed production of free and bound coagulases, production of acetion, anaerobic utilization of mannitol with acid production. PCR-amplified coagulase genes of S. aureus revealed different pattern in base pair lengths and number of amplified bands. There were no obvious specific PCR pattern for all types of isolates thus, genotypic clustering correlated to human, animal and environmental isolates was not passable. Given the specificity of the coagulase gene, the isolates were thus confirmed to be belong to the coagulase positive S. aurueus. Out of 29 PCR-amplification isolates, 21 produced a single band while 8 isolates produced two bands. The length of the amplicon ranged from 430 to 1000 bp. Amplicons of the 21 isolates were thus categorized as 670, 930, 950 and 1000 bp. In conclusion, Amplification of coagulase gene is useful in confirmation of coagulase gene positive S. aurueus.]]>Brunner, D.W. and J.H. Gillespie,19736th Edn.,Harvey, J. and A. Gilmour,1988Staphylococci from goats milk product produced in Northern Island.]]>77982Adlan, A., A.M. Shommein and E.D.M.E. Amin,198023739Farah, I.O.,1991303244Mamoun, I.E. and M.R. Bakhiet,1992Staphylococcal mastitis in the Sudan.]]>316264Mamoun, I.E.,19811981Bakhiet, M.R., J. Ahmed and E.D.M.E. Amin,1991Staphylococcus aureus from food poisoning of cheese.]]>39459459Goh, S.H., S.K. Byrne, J.L. Zhang and A.W. Chow,1992Staphylococcus aureus on the basis of coagulase gene polymorphism.]]>3016421645Yates, M.D., F.A. Drobniewski and S.M. Wilson,2002Mycobacterium tuberculosis.]]>40712714Nimmo, G.R., J. Schooneveldt, G. O'Kane, B. McCall and A. Vickery,2000Staphylococcus aureus in Southeast Queensland, Australia.]]>3839263931Newton, C.R. and A. Graham,19972nd Edn.,Elmagboul, S.B.,20012001Wilson, I.G.,19976337413751Chou, Q., M. Russel, D.E. Birch, J. Raymond and W. Bloch,19921617171723