Pakistan Journal of Biological Sciences 1028-8880 1812-5735 Asian Network for Scientific Information 10.3923/pjbs.2004.312.317 Aspergillus funiculosus G. Smith and its Enzyme Protease]]> Shumi W. Hossain Md. Towhid Anwar M. N. 3 2004 7 3 In the present study fungal colonies were isolated from different protein sources by enrichment culture technique and the fungal isolates were screened for protease activity. Isolate G, the most potent producers of protease were identified as Aspergillus funiculosus G. Smith. The isolate G was found to show highest protease production (activity) in an optimized medium (Yeast extract 0.5%, lactose 0.1% and KNO₃ 1%) at pH 7.0, temperature 37°C and 5 days of incubation period. The crude enzyme of the isolate G was found to show its highest activity at pH 7.5 and temperature 35°C in enzyme substrate reaction. The molecular weight of enzyme of the isolate Aspergillus funiculosus (G) was found 51 kDa, which was determined by SDS-PAGE technique.

]]> 1 Trevan, M.D., 1987 Enzyme Production. pp: 155-225 pp: 155-225 2 Kumar, H.D., 1998 A Text Book of Biotechnology. 173 173 3 Fermor, T.R. and D.A. Wood, 1981 Agaricus bisporus and other fungi.]]> Gen. Microbiol., 126 377 387 4 Rao, M.B., A.M. Tanksale, M.S. Ghatge and V.V. Deshpande, 1998 Microbiol. Mol. Biol. Rev., 62 597 635 5 Alvarez-sanchez, M.E.A., L. Avila-Gonzalez, C. Becerril-Garcia, L.F. Fattel-Facenda, J. Ortega-Lopes and R. Arroyo, 2000 Microbiol. Pathogenesis, 28 193 202 6 Gilman, J.C., 1957 A Manual of Soil Fungi. pp: 220 pp: 220 7 Matta, H., V. Punj and S.S. Kanwar, 1997 Pseudomonas sp. AFT-36 of dairy origin.]]> Applied Microbiol., 25 300 302 8 Hayashi, L.D., D. Fukushima and K. Mogi, 1967 Cited from Physiology and Parasitism. pp: 194 pp: 194 9 Meyers, S.P. and D.G. Ahearn, 1977 Mycologia Cited from Physiology and Parasitism. pp: 195 pp: 195 10 Mahadevan, A. and R. Sridhar, 1982 Methods in Physiological Plant Pathology. pp: 296 pp: 296 11 Laemmli, U.K., 1970 Nature, 227 680 685 12 Micales, J.A., 1992 Postia placenta.]]> Mycologia, 84 815 822 13 Suseela, R., 1998 Penicillium species.]]> Proceedings of the International Symposium on Ecology of Fungi, 1998 pp: 403 206 14 Sabita, R.R., M.M. Rahman and N. Choudhury, 1995 Saccharomyces cerevisiae strain DSM 1848.]]> Bangladesh J. Microbiol., 12 45 49 15 Marzan, L.W., M.A. Manchur, M.T. Hossain and M.N. Anwar, 2002 Fusarium poae. ]]> Bangladesh J. Microbiol., 18 127 134 16 Matsushima, K., M. Hayakawa, M. Ito and K. Shimada, 1981 J. Gen. Applied Microbial., 27 423 426 17 North, M.J., 1982 Microbiol. Rev., 46 308 340 18 Jawahar, L.D, A.K. Dutta and A.K. Bishwas, 1991 Nigella sativa L.]]> Bangladesh J. Bot., 20 117 123 19 Hossain, M.T., S. Akter, M.S. Rahman, M.A. Manchur and M.N. Anwar, 1999 Chittagong Univ. J. Sci., 23 101 106