Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2004.269.272in vitro Seedling of Tomato (Lycopersicon esculentum L., c.v. Roma)]]>ChaudhryZubeda HabibDarima RashidHamid QureshiAsfari Sharif2200472The in vitro response of Roma was tested using hypocotyls and leaf discs as an explant source for callus induction and regeneration. Leaf discs and hypocotyl showed variable response by use of different growth regulators. Formation of calli from leaf discs and hypocotyls were obtained on MS medium with IAA 2 mg l
-1, BAP 2 mg l-1, NAA 2 mg l-1 and kinetin 4 mg l-1. Callus formation was 82.5% from hypocotyl and 57% from leaf discs. Maximum percentages of shoot formation from hypocotyl and leaf discs derived calli was 45.8% and 30.8%, respectively on MS medium with IAA 2 mg l-1, BAP 5 mg l1, NAA 2 mg l-1 and kinetin 4 mg l-1.]]>Newman, P.O., S. Krishnasaj and P.K. Saxena,1996Lycopersicon esculentum L.) somatic embryogensis and shoot organogene from hypocotyl segments induced with Benzylaminopurine.]]>157554560Costa, M.G.C., F.I.S. Nogueira, M.L. Figueira, W.C. Otoni, S.H. Brommonschenkel and P.R. Cecon,2000Lycopersicon esculentum L.) cultivars.]]>19327333Chaudary, Z., I. Feroz, W. Ahmed, H. Rashid, B. Mirza and A. Quraishi,2001Lycopersicon esculentum L. to callogenesis and regeneration.]]>111381140Murashige, T. and F. Skoog,196215473497Davis, D.G., K.A. Breiland, D.S. Frear and G.A. Secor,1994Lycopersicon esculentum L.) cultivars with different sensitivities to meterbuzim, Plant growth.]]>226773Hill, T., M. Koornneef, M.S. Remanna and P. Zabel,198942123Duzvaman, E., A. Taurisever, G. Gunver, K.E. Cockskull, Y. Tuzel and A. Gul,1994in vitro.]]>366235242