Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2004.1636.1639SallehMohamad Ramlan Bin MohamedFaroukAbd. ElaziemSallehRohani BintiHasimHaslinda Binti9200479The purpose of this study was to determine the effect of operating parameters
such as the yeast concentration, processing temperature and isolation time on
the isolation of GSH using Homogenizer Method. The disrupted cells solution was
centrifuged at 12,000 rpm for 20 min and the GSH content in the supernatant was
analyzed by taking the optical density (OD) value of the solution using Spectrophotometer
at 412 nm. Yeast solution with the concentration of 3, 6, 9 and 12 wt.% was disrupted
at their original temperature for 30 sec to study the effect of yeast concentration.
The result obtained shows that the optimum value of yeast concentration is 9 wt.%
where 10.98 μmol mL-1 GSH was isolated. To study the effect of
processing temperature, isolation process of the 9 wt.% yeast solution was done
at various values of temperature such as 19, 22, 25, 28, 30 and 40°C for 30 sec.
The results indicated that, the optimum GSH isolation temperature is 22°C with
the isolation of 17.93 μmol mL-1 GSH. Then, to study the effect
of isolation time, the isolation process of sample with the concentration of 9
wt.% was done at 22°C for 15, 30, 45 and 60 sec. The result indicated that the
optimum isolation time for the Ultrasonic process is 30 sec.]]>Stephen, D.W. and D.J. Jamieson,1996Saccharomyces cerevisiae.]]>141207212Li, Y., J. Chen, Q. Song, Y. Katakura and S.Y. Lun,199713105113Shimizu, H., K. Araki, S. Shioya and K. Suga,199138196205Sakato, K. and H. Tanaka,199240904912Liu, C.H., C.F. Hwang and C.C. Liao,1999Saccharomyces cerevisiae.]]>341723Ohwada, T. and S. Sagisaka,1990Escherichia coli B caused by osmotic stress.]]>5433713372Alfafara, C., A. Kanda, K. Araki, H. Shimizu, S. Shioya and K. Suga,1992Saccharomyces cerevisiae.]]>36538540Alfafara, C.G., K. Miura, H. Shimizu, S. Shioya and K.I. Suga,1992Saccharomyces cerevisiae.]]>37141146Li, Y., J. Chen, N.D. Zhou, W. Fu, W. Ruan and S. Lun,199829537542Li, Y., J. Chen, N. Zhou, W. Fu, W. Ruan and S. Lun,199814147152Murata, K.,19821982Murata, K. and A. Kimura,1982Escherichia coli B.]]>4414441448Murata, K., T. Miya and H. Gushima,1983Escherichia coli B.]]>4713811383Wei, G., Y. Li, G. Du and J. Chen,2003Saccharomyces cerevisiae.]]>3811251258Shi, L., W. Bochu, L. Zhiming, D. Chuanren, D. Chuanyun and A. Sakanishi,2003S. cerevisiaes.]]>304349Ramlan, M., H. Yumi-Zuhanis, R.B. Salleh and H.B. Hasim,2004715461549Hopkins, D.L., L. Martin and A.R. Gilmour,200467705710