Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2000.842.845Cicer arietinum L.)]]>HusnainTayyab Tahira Fatima Rafi-ul-Islam Sheikh Riazuddin 5200035In order to develop an efficient protocol for inserting novel genes in chickpea plants, conditions of regeneration and transformation of tissues were optimized. In case of in vitro plant regeneration of Cicer arietinum variety 6153, the hypocotyl tissue showed somatic embryogenesis and regenerated shoots in two basal media with addition of NAA and BAP. Different auxins seemed to have different effects on the differentiation and maturation of embryos. The replacement of one auxin NAA with another IAA induced the germination of embryos. Elaborate rooting of regenerated shoots in two basal media containing IBA was obtained with a frequency of 80 percent. Conditions of biolistic transformation were also optimized for transient expression of marker gene using a homemade particle acceleration gun. The beta-glucuronidase gene was introduced into hypocotyl tissue for transient expression and upto 58 percent of hypocotyl showed transient expression. These results provide the basic information for the transformation of chickpea via somatic embryogenesis.]]>Altaf, N. and A.S. Ahmed,1986Cicer arietinum L.) through tissue culture techniques.]]>1986pp: 407417Barna, K.S. and A.K. Wakhlu,1993Cicer arietinum L.).]]>12521524Bhojwani, S.S. and A. Mukhopadhyay,19841984pp: 373-375pp: 373-375Draper, J., R. Scott, P. Armitage and R. Walden,1988pp: 96-98pp: 96-98Gharyal, P.K. and S.C. Maheshwari,1983Lathyrus sativus.]]>66123126Ghazi, T.D., H.V. Cheema and M.W. Nabors,1986Glycine max L.]]>5452456Gosal, S.S. and Y.P.S. Bajaj,19796155160Husnain, T., T. Malik, S. Riazuddin and M.P. Gordon,199749716Jefferson, R.A.,19875387405Kumar, A.S., T.P. Reddy and G.M. Reddy,1983Cajanus cajan L.).]]>32271278Kumar, A.S., O.L. Gamborg and M.W. Nabors,1988Vigna aconitifolia.]]>7138141Kumar, V.D., P.B. Kirti, J.K.S. Sachan and V.L. Chopra,1994Cicer arietinum L.).]]>13468472Meijer, E.G.M. and D.C.W. Brown,1987Medicago sativa.]]>69591596Myers, J.R., P.A. Lazzeri and G.B. Collins,1989Glycine species from suspension culture-derived protoplasts.]]>8112115Riazuddin, S., T. Husnain, T. Malik, H. Farooqi and S.T. Abbar,19889339345Sagare, A.P., K. Suhasini and K.V. Krishnamurthy,1993Cicer arietinum L.).]]>12652655Saxena, P.K. and J. King,1987Lens culinaris medik. via somatic embryogenesis.]]>52223227Vain, P., N. Keen, J. Murillo, C. Rathus, C. Nemes and J.J. Finer,199333237246