Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2000.819.821In vitro Production of Cormlets in Gladiolus]]>AhmadTauqeer Saeed AhmadMuhammad Ahmad NasirIdrees RiazuddinSheikh 5200035The ability of explants of gladiolus cv White Prosperity to form plantlets and cormlets was investigated in vitro studies. Maximum production of shoots per explant was obtained on basal medium of Murashige and Skoog (1962) containing 18 μM 6-Benzylaminopurin (BAP) and 36 μM Indole-3-butyric Acid (IBA). Callus formation was observed when in vitro cultures were maintained for 16-18 weeks. When different explants were used for their response to regenerate, maximum number of shoots per culture was achieved from nodal buds. In order to allow initiation of root formation, the shoots were transferred to one half strength Murashige and Skoog medium containing 2.5 μM, Naphthalenacetic Acid (NAA) or 19 μM Indole -3-butyric Acid (IBA). Roots developed readily within 4-5 weeks on either of the two media. However, all plantlets grew with better survival in medium containing NAA as harmonal supplement than in media containing IBA. Further MS medium containing 6 percent sucrose facilitated maximum growth of cormlets to allow their use as propagation material in vivo.]]>Arora, J.S., K. Singh, H.S. Grewal, S.S. Gosal and Y.R. Chanana,1996In vitro Cormel Production from Nodal Buds and Cormel Tips in Gladiolus.]]>1996pp: 50-53pp: 50-53Bajaj, Y.P.S.,1983pp: 18-42pp: 18-42Dantu, P.K. and S.S. Bhojwani,1987In vitro propagation and corm formation in gladiolus.]]>529093De Bruyn, M.H. and D.I. Ferreira,1992In vitro corm production of Gladiolus dalenii and G. tristis.]]>31123128Grewal, H.S., S.S. Gosal, J.S. Arora and K. Singh,19901990Hoidgate, D.P.,19771977pp: 18-42pp: 18-42Hussey, G.,1977In vitro propagation of Gladiolus by precocious axillary shoot formation.]]>6287296Lilien-Kipnis, H. and M. Kochba,1987164169180Logan, A.E. and F.W. Zettler,1984in vitro propagation of virus-indexed gladioli.]]>164169180Murashige, T. and F. Skoog,196215473497Nasir, I.A., H. Afrasiab and S. Riazuddin,1996in-vitro technique.]]>4165169Stefaniak, B.,1994Gladiolus hort.).]]>13386389Steinitz, B., A. Cohen, Z. Goldberg and M. Kochba,1991266370Ziv, M., A.H. Halevy and R. Shilo,197034671676Ziv, M.,1979Gladiolus plants propagated in vitro.]]>11257260