Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2000.2226.2228NoorSamina HusnainTayyab RiazuddinSheikh 122000312A simple leaf tip assay was used for screening of putative transgenic plants expressing the hygromycin resistance gene (hph) or kanamycin resistance gene (nptll). Leaf tips were excised from in vitro or in vivo transgenic plants and cultured on MS medium without phytohormons containing the suitable concentrations of hygromycin and kanamycin. Leaf tips of plants transformed with the marker gene showed no significant effect of the appropriate drug for at least two weeks. While non-transgenic leaf tips had noticeable symptoms of bleaching, necrosis or browning after 3-4 days of selection. This method is simple, rapid and allows clear distinction between transformed and non-transformed plants both, in monocots and dicots.]]>Blochlinger, K. and H. Diggelmann,1984429292931Datta, S.K., K. Datta, N. Soltanifar, G. Donn and I. Potrykus,199220619629Gritz, L. and J. Davies,1983Escherichia coli and Saccharomyces cerevisiae.]]>25179188Hiei, Y., S. Ohta, T. Komari and T. Kumashiro,1994Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.]]>6271282Husnain, T., F. Khanum, S. Riazuddin and M.P. Gordan,1995Oryza Sativa L.) with bacterial genes by particle bombardment.]]>1219228Jefferson, R.A., S.M. Burgess and D. Hirsh,1986Escherichia coli as a gene-fusion marker.]]>8384478451Murashige, T. and F. Skoog,196215473497Uchimiya, H., T. Fushimi, H. Hashimoto, H. Harada, K. Syono and Y. Sugawara,1986Oryza sativa L.).]]>204204207Haris, W.A.A., S. Noor, T. Hussain and S. Riazuddin,1999Gossypium hirsutum L.) by particle bombardment.]]>2804806Haris, W.A.A., T. Husnain and S. Riazuddin,1998Gossypium hirsutum L.) with insect resistant gene by particle bombardment and agrobacterium.]]>1170174