Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2000.1601.1604Oryza Species]]>AsgharMuhammad BrarDarshan S. 102000310For molecular characterization of F1, hybrid of O. sativa×0. officinalis and its backcross-1 generation (8C1) with 0. sativa through genomic in situ hybridization (GISH), biotin labelled total genomic DNA from O. officinalis was used as probe. Cytological preparations were made by enzymatic maceration technique. Probe was hybridized onto chromosomal preparations at 37°C and signals were detected by colorimetric method using 3-amino-9-ethylcarbazole. Labelling efficiency of probe was determined by dot blot method prior to hybridization reaction. Based on the appearance of signal on chromosomes, it was inferred that there exists partial homoeology between the genomes of 0. sativa and O. officinal and there are higher chances of gene(s) transfer from O. officinalis to O. sativa. More over the study shows that GISH is a powerful technique for genomic characterization of breeding material at any generation.]]>Abbasi, F.M., D.S. Brar, A.L. Carpena, K. Fukui and G.S. Khush,1998O. saliva L. x O. australiensis Domin through genomic in situ hybridization.]]>158485Abbasi, F.M., D.S. Brar, A.L. Carpena and G.S. Khushu,1998O. saliva L. x O. brachyantha A. Chev. et Roehr. derivatives.]]>158687Asghar, M., D.S. Brar, J.E. Hernandez, N. Ohmido and G.S. Khush,1998Oryza sativa L. and O. officinalis Wall ex Watt. through fluorescence in situ hybridization.]]>158384Bennett, S.T., A.Y. Kenton and M.D. Bennett,1992in situ hybridization reveals the allopolyploid nature of Milium montianum (Gramineae).]]>101420424Chen, Q. and K. Armstrong,1994in situ hybridization in Avena sativa.]]>37607612Dellaporta, S.L., J. Wood and J.B. Hicks,198311921Endo, T.R., Y. Mukai, M. Yamamoto and B.S. Gill,199166291295Fukui, K., R. Shishido and T. Kinoshita,1997in situ hybridization.]]>9512391245Gall, J.G. and M.L. Pardue,196963378383Le, H.T., K.C. Armstrong and B. Miki,19897150158Iqbal, N.,1998Aeghops unillfistata chromoaomas and the transfer of aluminium tolerance to wheat by induced recombination.]]>1998Iwano, M., K. Sakamoto, G. Suzuki, M. Watanabe and S. Takayama et al.,1998Brassica campestris L. by multicolor FISH.]]>96751757John, H.A., M.L. Birnstiel and K.W. Jones,1969223582587Langer-Safer, P.R., M. Levine and D.C. Ward,1982Drosophila polytene chromosomes.]]>7943814385Leitch, A.R., W. Mosgoller, T. Schwarzacher, M.D. Bennett and J.S. Heslop-Harrison,1990in situ hybridization to sectioned nuclei shows chromosome domains in grass hybrids.]]>95335341Manuelidis, L., P.R. Langer-Safer and D.C. Ward,198295619625Mujeeb-Kazi, A., M.N. Islam-Faridi and A. Cortes,1996Triticeae species intergeneric hybrids by fluorescent in situ hybridization.]]>61307315Mukai, Y., B. Friebe and B.S. Gill,1992in situ hybridization sites using highly repetitive and total genomic rye DNA probes of Imperial rye chromosomes added to Chinese spring wheat.]]>677183Mukai, Y., Y. Nakahara and M. Yamamoto,1993in situ hybridization using total genomic and highly repeated DNA probes.]]>36489494Parokonny, A.S., A.Y. Kenton, Y.Y. Gleba and M.D. Bennett,1992Nicotiana asymmetric somatic hybrids analysed by in situ hybridization.]]>2863874Rayburn, A.L. and B.S. Gil,1986767881Scherthan, H., M. Kohler, P. Vogt, K. Von Malieh and D. Schweizer,1992in situ hybridization with doubs e-labeied DNA: Signal amp Uflcation at the probe tevel.]]>6047Schwarzacher, T., A.R. Leitch, M.D. Bennett and J.S. Heslop-Harrison,1989In situ localization of parental genomes in a wide hybrid.]]>64315324Schwarzacher, T., K. Anamthawat-Jonsson, G.E. Harrison, A.K.M.R. Islam and J.Z. Jia et al.,1992in situ hybridization to identify alien chromosomes and chromosome segments in wheat.]]>84778786Singer, R.H. and D.C. Ward,19827973317335