Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2000.1313.1314AkhtarMasood AhmedRehan HayatC. S. HussainI. AshfaqueM. 8200038Immune response of formalin inactivated and binnary ethyleneimine inactivated Angara disease (hydropericardium) vaccines were compared using indirect hemagglutination (IHA) test. A four fold increase in IHA antibody titre was recorded with binnary ethyleneimine (BEI) inactivated vaccine. Agar gel diffusion test gave a strong precipitation line with serum of chicks vaccinated with BEI inactivated vaccine whereas a weak precipitation line was observed with serum from chicks vaccinated with formalin inactivated vaccine. The results of challenge test were promising and no adverse effects were seen in vaccinated chicks. BEI as inactivating agent produced antigenically superior vaccine.]]>Afzal, M. and I. Ahmad,19901265960Ahmad, I., M.I. Malik, K. Iqbal, K. Ahmed and S. Naz,199060131138Cheema, A.H., I. Ahmad and M. Afzal,19898789795Chishti, M.A., M. Afzal and A.H. Cheema,19898797801Hassan, N.U., M. Ifzal, A. Hameed and R.A.R. Khan,199414510Hussain, I., A.A. Anjum and I. H. Khan,199616125127Naeem, K., M. Rabbani, M. Hussain and A.H. Cheema,199515150151Rahman, S.U., M. Ashfaque, A.D. Anjum and T.A. Sinfhu,19891989pp: 7374Brown, F. and J. Crick,195982444447