Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.1998.11.14G. barbadense genes into G. hirsutum through DNA-mediated Embryo Transformation approach]]>AslamM. M. T. Elahi N. Iqbal 1199811Attempts were made to incorporate intraploid interpsecific gene(s) from Gossyium barbadense into G. hirsutum through DNA-mediated embryo transformation approach. G. barbadense DNA was injected into the styles/ovaries of G. hirsutum, 24 hours after self pollination. Two phenotypic types of plants i.e. transformed and G. hirsutum like were observed in D population. The transformed plants had clearcut variations in boll weight, yield and other quality traits confirmed to recipient parent. D transformations were also heritable and were transmitted to D generation. The transformed plants had better boll weight (4.9 gm), better fibre fineness (3.9 μg/in) and better fibre strength (101.0 TPPSI) compared to the boll weight (3.0 gms), fibre fineness (4.5 μg/in) and fibre strength (91.0 TPPSI) of G. hirsutum. The transformation efficiency for quantitative traits was about 20 % where as low for qualitative traits like flower petals with red spot (0.08 %). In the present studies transformed genotypes illustrated higher yield potential and better fibre quality than the recipient (G. hirsutum) parent.]]>Aslam, M., M.T. Elahi and N. Iqbal,1995In vivo incorporation of intraploid intraspecific genes into cotton, G. hirsutum, via embryo transformation.]]>1209218Beasley, J.O. and M.S. Brown,194265421427Bryan, W.E.,1955766Crossway, A., J.W. Oakes, J.M. Irvine, B. Ward, V.C. Knauf and C.K. Shewmaker,1986202179185De la Pena, A., H. Lorz and J. Schell,1987325274276Finer, J.J. and M.D. McMullen,1990Gossypium hirsutum L.) via particle bombardment.]]>8586589Fromm, M., L. Taylor and V. Walbot,19858258245828Gawel, N.J. and C.D. Robacker,199049249253Hess, D.,1969Petunia hybrida.]]>60348353Holl, F.B.,19751975Horsch, R.B., J.E. Fry, N.L. Hoffman, D. Eichholtz, S.A. Rogers and R.T. Fraley,198522712291232Howland, G.P., R.W. Hart and M.L. Yette,1975278187Kohler, F., G. Cardon, M. Pohlman, R. Gill and O. Schieder,198912189199Korohoda, J. and K. Strzalka,1979949599Leonard, G.D., M.D. Dibner and J.F. Batty,19861986pp: 47-50pp: 47-50Potrykus, I.,199142205225Neuhaus, G., G. Spangenberg, O.M. Scheid and H.G. Schweiger,1987753036Shillito, R.D., M.W. Saul, J. Paszkowski, M. Muller and I. Potrykus,1985310991103Zhuaib, M., K.A. Siddiqui, M. Salahuddin and S. Hoda,1981183742Soyfer, V.N.,198058225235Stephens, S.G.,1949Gossypium. I. Selective elimination of the donor parent genotype in interspecific backcrosses.]]>34627637Stephens, S.G.,197461128141Trolinder, N.L. and C. Xhixian,19898133136Werner, C.P. and M.A. Cornish,1985Nicotiana rustica by means of irradiated pollen IV. Qualitative characters.]]>55315320Zhou, G., J. Weng, J. Huang, S. Qian and G. Liu,19821982Zhou, G., J. Weng, Y. Zeng, J. Huang, S. Qian and G. Liu,1983101433481