Pharmacologia2044-46482044-4656Science International10.3923/pharmacologia.2016.182.192TamokouJean-de-Dieu TsemeugneJoseph FondjoEmmanuel Sopbue SarkarProdipta KuiateJules-Roger DjintchuiArnaud Ngongang SondengamBeibam Luc BagPrasanta Kumar 4201674Objective: The emergence of resistance to the major classes of antibacterial drugs is recognized as a serious health concern. Chemotherapy of cervical cancer, a devastating cancer with increasing worldwide incidence and mortality rates is largely ineffective. The discovery and development of effective antibacterial/anticancer agents is urgently needed. The present study reports on the evaluation of antibacterial and anticancer properties of some azo compounds containing thiophene backbone. Methodology: The antibacterial activity of the synthesized compounds was assessed by performing Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and the time-kill kinetic study, while the cytotoxic activity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay using HeLa (cancer cell lines) and Vero (normal) cells. Results: All of the compounds tested here showed significant antibacterial activity against the strains of Gram-positive bacteria, Staphylococcus aureus and Gram-negative multi-drug resistance bacteria, Vibrio cholerae (causative agent of cholera) and Shigella flexneri (causative agent of shigellosis), as well as the significant cytotoxic activity (LC50, 1.03-106.73 μg mL1) against HeLa cells. 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