Pharmacologia2044-46482044-4656Science International10.3923/pharmacologia.2015.377.385In-vitro Free Radicals Scavenging and Antioxidant Activity of Rice Bran Extract]]>MuthalAmol Pramod RojatkarSupada Rambhau BodhankarSubhash Laxmanrao 8201568Aim: To evaluate the antioxidant activities of the γ-oryzanol isolated from rice bran oil of Indian origin in various in vitro models. Materials and Methods: The hydrogen donating ability of the hydroalcoholic extract of γ-oryzanol was measured in the presence of 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical. Results: A 100 μg mL-1 of γ-oryzanol and ascorbic acid exhibited 64.56 and 64.88% inhibition, respectively and the IC50 values were found to be 48 and 56.50 μg mL-1 for γ-oryzanol and ascorbic acid respectively. The effect of γ-oryzanol on reducing power was studied according to the reaction of Fe+3 to Fe+2. The reducing power of the extract increased with the increasing amount of the concentration. The total polyphenolic content of the γ-oryzanol was tested using Folin-Ciocalteau reagent. It was found that γ-oryzanol contained 19.31±1.79 mg of GA/g, which is significant (p<0.05) when compared to gallic acid. γ-oryzanol hydrogen peroxide (H2O2) radical at IC50 values of 45 μg mL-1 against the corresponding standards ascorbic acid (IC50 = 35 μg mL-1). Moreover, the results were observed in a concentration dependent manner. Conclusion: All the above in-vitro studies clearly designate that the hydroalcoholic isolated γ-oryzanol has a significant antioxidant activity.]]>Adil, M., A. Visnagri, V.S. Kumar, A.D. Kandhare, P. Ghosh and S.L. Bodhankar,20145222234Aswar, U.M., A.D. Kandhare, V. Mohan and P.A. Thakurdesai,201529423433Badole, S.L., S.M. Chaudhari, G.B. Jangam, A.D. Kandhare and S.L. Bodhankar,2015Pongamia pinnata in streptozotocin-nicotinamide induced diabetic rats.]]>2015Bhalsinge, R.R., P.S. Worlikar, A.V. Tilak, S.L. Bodhankar and P. Ghosh et al.,20122556563Kandhare, A.D., S.L. Bodhankar, V. Mohan and P.A. Thakurdesai,201524111Valko, M., C.J. Rhodes, J. Moncol, M. Izakovic and M. Mazur,2006160140Ghosh, P., A.D. Kandhare, D. Gauba, K.S. Raygude and S.L. Bodhankar,2012Helicobacter pylori infected acid peptic disease patients.]]>2S783S789Ghosh, P., A.D. Kandhare, V.S. Kumar, A.R. Rajmane, M. Adil and S.L. Bodhankar,20122012S671S678Ghosh, P., A.D. Kandhare, K.S. Raygude, D. Gauba, T.P. Gosavi and S.L. Bodhankar,2012H. pylori infection: A meta-analysis of literature.]]>4128134Ridnour, L.A., J.S. Isenberg, M.G. Espey, D.D. Thomas, D.D. Roberts and D.A. Wink,20051021314713152Kovacic, P. and J.D. Jacintho,20018773796Ghosh, P., A.D. Kandhare, K.S. Raygude, V.S. Kumar, A.R. Rajmane, M. Adil and S.L. Bodhankar,20122012S642S650Gosavi, T.P., P. Ghosh, A. Kandhare and S.L. Bodhankar,20111894906Gosavi, T.P., A.D. Kandhare, K.S. Raygude, P. Ghosh and S.L. Bodhankar,2011Viscum album and Rauwolfia serpentina mother tincture in hypertensive patients.]]>22935Gosavi, T.P., P. Ghosh, A.D. Kandhare, V.S. Kumar, M. Adil, A.R. Rajmane and S.L. Bodhankar,2012H. pylori nosode, a homeopathic preparation in healing of chronic H. pylori infected ulcers in laboratory animals.]]>2S603S611Gosavi, T.P., A.D. Kandhare, P. Ghosh and S.L. Bodhankar,2012Argentum metallicum, a homeopathic preparation.]]>4626637Gosavi, T.P., A.D. Kandhare, K.S. Raygude, P. Ghosh and S.L. Bodhankar,201121030Goswami, S., A. Kandhare, A.A. Zanwar, M.V. Hegde and S.L. Bodhankar et al.,20142014Honmore, V., A. Kandhare, A.A. Zanwar, S. Rojatkar, S. Bodhankar and A. Natu,2015Artemisia pallens alleviates acetaminophen induced toxicity via modulation of endogenous biomarkers.]]>53571581Kamble, H., A.D. Kandhare, S. Bodhankar, V. Mohan and P. Thakurdesai,20133145151Kandhare, A.D., M.V. Patil and S.L. Bodhankar,201537709721Kandhare, A.D., K.S. Raygude, P. Ghosh and S.L. Bodhankar,20115236243Kandhare, A.D., J. Alam, M.V.K. Patil, A. Sinha and S.L. Bodhankar,20152015Kandhare, A.D., S.L. Bodhankar, V. Mohan and P.A. Thakurdesai,201553545Kandhare, A.D., S.L. Bodhankar, V. Mohan and P.A. Thakurdesai,201572323334Kandhare, A.D., S.L. Bodhankar, V. Mohan and P.A. Thakurdesai,2015237151165Kandhare, A.D., S.L. Bodhankar, V. Singh, V. Mohan and P.A. Thakurdesai,201332330Kandhare, A.D., P. Ghosh and S.L. Bodhankar,20132013Kandhare, A.D., P. Ghosh and S.L. Bodhankar,2014219101112Kandhare, A.D., P. Ghosh, A.E. Ghule and S.L. Bodhankar,201327603622Kandhare, A.D., P. Ghosh, A.E. Ghule, G.N. Zambare and S.L. Bodhankar,2013Phyllanthus amarus by modulation of endogenous biomarkers and DNA damage in acetic acid induced ulcerative colitis: Role of phyllanthin and hypophyllanthin.]]>108797Kandhare, A.D., V.S. Kumar, M. Adil, A.R. Rajmane, P. Ghosh and S.L. Bodhankar,2012Xanthium strumarium L. by modulation of cellular and biochemical marker.]]>12287299Kandhare, A.D., K.S. Raygude, P. Ghosh, A.E. Ghule and S.L. Bodhankar,201283650659Kandhare, A.D., K.S. Raygude, P. Ghosh, A.E. Ghule and S.L. Bodhankar,20125111822Kandhare, A.D., K.S. Raygude, P. Ghosh, A.E. Ghule, T.P. Gosavi, S.L. Badole and S.L. Bodhankar,2012 Hibiscus rosa sinensis Linn. leaves in experimental colitis in rats.]]>2337344Kandhare, A.D., K.S. Raygude, P. Ghosh, T.P. Gosavi and S.L. Bodhankar,201137183Kandhare, A.D., K.S. Raygude, P. Ghosh, T.P. Gosavi and S.L. Bodhankar,2011210241032Kandhare, A.D., K.S. Raygude, V.S. Kumar, A.R. Rajmane and A. Visnagri et al.,20122173186Kandhare, A.D., V. Shivakumar, A. Rajmane, P. Ghosh and S.L. Bodhankar,201468586603Droge, W.,2002824795Ketkar, S., A. Rathore, A. Kandhare, S. Lohidasan, S. Bodhankar, A. Paradkar and K. Mahadik,20154147160Kumar, V.S., A.R. Rajmane, M. Adil, A.D. Kandhare, P. Ghosh and S.L. Bodhankar,201428132145Patil, M.V.K., A.D. Kandhare and S.D. Bhise,2011Cucumis sativus L. fruit formulation on wound healing in Wistar rats.]]>2207213Patil, M.V.K., A.D. Kandhare and S.D. Bhise,2012Daucus carota root on experimental colitis in rats.]]>4337343Patil, M.V.K., A.D. Kandhare and S.D. Bhise,2012Xanthium srtumarium L. ethanolic extract in freund's complete adjuvant induced arthritis.]]>2615Patil, M.V.K., A.D. Kandhare and S.D. Bhise,2012Cucumis sativus Linn. fruit in ulcerative colitis in laboratory animals.]]>2S962S969Patil, M.V.K., A.D. Kandhare and S.D. Bhise,2012Daucus carota Linn root formulated cream on wound healing using excision and incision wound model.]]>2S646S655Patil, M.V.K., A.D. Kandhare, P. Ghosh and S.D. Bhise,2012Fragaria vesca L. ethanolic extract in chemically induced epilepsy in laboratory animals.]]>12255264Raygude, K.S., A.D. Kandhare, P. Ghosh, T.P. Gosavi and S.L. Bodhankar,2011H. pylori infection: A cumulative meta-analysis of literature.]]>1338345Raygude, K.S., A.D. Kandhare, P. Ghosh and S.L. Bodhankar,20122215222Raygude, K.S., A.D. Kandhare, P. Ghosh, A.E. Ghule and S.L. Bodhankar,201220331341Saraswathi, K.Y., A. Muthal, A. Kandhare, S. Rojatkar and S. Bodhankar,2014Artemisia pallens wall on endurance of laboratory animals.]]>5298309Ji, L.L., 1999222283292Sarkar, S., A. Sengupta, A. Mukhrjee, A. Guru, A. Patil, A.D. Kandhare and S.L. Bodhankar,20156273281Shivakumar, V., A.D. Kandhare, A.R. Rajmane, M. Adil and P. Ghosh et al.,201476174178Visnagri, A., A.D. Kandhare and S.L. Bodhankar,201537482493Visnagri, A., A.D. Kandhare, S. Chakravarty, P. Ghosh and S.L. Bodhankar,201452814828Sen, C.K.,199579675686Ramarathnam, N., T. Osawa, M. Namiki and S. Kawakishi,198937316319Kim, J.S., J. Godber, J. King and W. Prinyawiwatkul,200178685689Muthal, A.P., S.R. Rojatkar and S.L. Bodhankar,20146354360Visnagri, A., A.D. Kandhare, P. Ghosh and S.L. Bodhankar,201328597Visnagri, A., A.D. Kandhare, V.S. Kumar, A.R. Rajmane and A. Mohammad et al.,20122157172