Pharmacologia2044-46482044-4656Science International10.3923/pharmacologia.2013.391.399Wattakaka volubilis Leaf Petroleum Ether Cold Macerated Extract Against Experimentally Induced Diabetes in the Rat]]>GopalVelmani MandalVivekananda MandalSubhash C. 5201345Objective: Wattakaka volubilis is widely used in Indian traditional
medicine to treat pain, cough, fever, dyspepsia and diabetes. This study explores
the antidiabetic potential of Petroleum Ether Cold Maceration Extract (PEME)
of Wattakaka volubilis in alloxan induced diabetes in rats. Materials
and Methods: The Petroleum Ether Cold Macerated Extract (PEME) of W.
volubilis was evaluated to quantify lupeol by HPTLC method. Male Wistar
rats were divided into five groups (with six rats each) and fed at libitum:
the control (0.9% saline), alloxan treated rats with or without supplementary
PEME of W. volubilis and metformin (50,100 and 250 mg kg-1
b.wt.) for three weeks. The blood-glucose, α-amylase, Alanine Transaminase
(ALT), Alkaline Phosphatase (ALP) and bilirubin levels were measured on 7, 14
and 21 day of PEME treatment on alloxan treated rats. Results: Histopathological
changes in the pancreas, kidney and liver were examined with hematoxylin-eosin
staining. The PEME had 382.82 μg of lupeol, treatment of PEME in experimental
rats by oral injections for 21 days showed reductions in the levels of serum
biochemical markers. Histopathology results showed that PEME administration
suppressed the abnormal cellular degenerations in alloxan treated rats. Conclusion:
These results suggest that PEME has a protective effect over alloxan-induced
diabetes.]]>American Diabetes Association,201033S62S69Andallu, B. and N.C. Varadacharyulu,2002Morus indica L.) leaves in streptozotocin diabetic rats.]]>40791795Wild, S., G. Roglic, A. Green, R. Sicree and H. King,20042710471053Nageswara Rao, T., C.T. Kumarappan, S. Mohana lakshmi and S.C. Mandal,2007Talinum portulacifolium (forssk) in alloxan-induced diabetic rats.]]>2407417Eurich, D.T., F.A. McAlister, D.F. Blackburn, S.R. Majumdar, R.T. Tsuyuki, J. Varney and J.A. Johnson,2007335497506Chhetri, D.R., P. Parajuli, G.C. Subba,200599199202Shirwaikar, A., K. Rajendran and R. Barik,2006Garuga pinnata Roxb. in streptozotocin-nicotinamide induced type-II diabetes mellitus.]]>107285290Panda, N., S. Banerjee, N.B. Mandal and N.P. Sahu,20061665669Ayyanar, M., K. Sankarasivaraman and S. Ignacimuthu,200812276280Akowuah, G.A., I. Zhari, I. Norhayati and A. Mariam,2006Andrographis paniculata.]]>19118126Trinder, P.,196922158161Kumarappan, C.T., T.N. Rao and S.C. Mandal,2007Ichnocarpus frutescens modifies hyperlipidemia status in diabetic rats.]]>6175187Narvaez-Mastache, J.M., M.L. Garduno-Ramirez, L. Alvarez and G. Delgado,2006Eysenhardtia platycarpa.]]>6916871691Nachnani, J.S., D.G. Bulchandani, A. Nookala, B. Herndon and A. Molteni et al.,201053153159Aughsteen, A.A. and F.I. Mohammed,2002in vivo study.]]>23838844Tarling, C.A., K. Woods, R. Zhang, H.C. Brastianos, G.D. Brayer, R.J. Andersen and S.G. Withers,20089433438Wannang, N.N., N.S. Jimam, S. Omale, M.L.P. Dapar, S.S. Gyang and J.C. Aguiyi,2007Cucumis metuliferus (Cucurbitaceae) fruits on enzymes and haematological parameters in albino rats.]]>625152518