Pharmacologia2044-46482044-4656Science International10.3923/pharmacologia.2012.490.498Alstonia scholaris Linn. R.Br.]]>ArulmozhiS. MazumderPapiya MitraSathiyanarayananL. ThakurdesaiPrasad A.102012310Background: The antianxiety activity of the ethanolic extract of leaves of Alstonia scholaris (Family: Apocynaceae) has been well established. The objective of the present study is to evaluate the anti-anxiety activity of the ethyl acetate fraction from ethanolic extract of leaves of Alstonia scholaris. Material and method: EA fraction from Alstonia scholaris was tested against various anti-anxiety models viz. elevated plus maze, open field, hole board, light dark, mirror chamber and foot shock induced aggression models. The change in brain monoamines was estimated. The possible serotonergic effect was tested by 5-Hydroxy Trypto Phan (5-HTP) induced wet dog shake, tail suspension and modified forced swim tests. Result: EA was found to be significantly active in open filed test, foot shock induced aggression and mirror chamber models of anxiety. However, lack of activity in elevated plus maze, light dark box, hole board test models, decrease in spontaneous motor activity, potentiation of 5-HTP, increase in 5-hydroxy tryptamine in brain indicate EA to have serotonergic effects. Decrease in immobility time in tail suspension test which was inhibited by reserpine and increase in swimming behaviour in forced swim test were conclusive evidence of selective serotonin reuptake inhibition. Conclusion: The present study suggest that EA possess antianxiety and antidepressant activity. The apparent mechanism of action is selective serotonin re-uptake inhibition.]]>Arulmozhi, S., P.M. Mazumder, P. Ashok, B. Hulkoti, L.S. Narayanan,2007Alstonia scholaris Linn. R.Br.]]>3106111Arulmozhi, S., P.M. Mazumder, P. Ashok and L. Sathiyanarayanan,2007Alstonia scholaris Linn.(Apocynaceae)-A review.]]>1163170Arulmozhi, S., P.M. Mazumder, S. Lohidasan and P.A. Thakurdesai,2010Alstonia scholaris Linn. R.Br.]]>22332Arulmozhi, S., P.M. Mazumder, L.S. Narayanan and P.A. Thakurdesai,2008Alstonia scholaris Linn.R.Br.]]>3761775Bedard, P. and C.J. Pycock,198816663670Costall, B. and R.J. Naylor,199511629892999Crawley, J. and F.K. Goodwin,198013167170Cryan, J.F. and I. Lucki,20002c receptors.]]>29511201126Cryan, J.F., A. Markou and I. Lucki,200223238245Cryan, J.F., R.J. Valentino and I. Lucki,200529547569Dhingra, D. and R. Valecha,2007Terminalia bellirica Roxb. fruits in mice.]]>45610616Dunham, N.W. and T.S. Miya,195746208209Fujishiro, J., T. Imanishi, J. Baba and K. Kosaka,200185327330Graeff, F.G., M.B. Viana and C. Tomaz,1993266770Handley, S.L., J.W. McBlane, M.A. Critchley and K. Njunge,199358203210Iwo, M.I., A.A. Soemardji, D.S. Retnoningrum and U.U.M. Sukrasno,2000Alstonia scholaris L. R.Br., Apocynaceae) bark extracts.]]>23177183Jain, N.N., C.C. Ohal, S.K. Shroff, R.H. Bhutada, R.S. Somani, V.S. Kasture and S.B. Kasture,2003Clitoria ternatea and the CNS.]]>75529536Keawpradub, N., G.C. Kirby, J.C.P. Steele and P.J. Houghton,1999Alstonia species from Thailand.]]>65690694Kirtikar, K.R. and B.D. Basu,2002Vol. 1,Kshama, D., H.J. Hrishikeshavan, R. Shanbhogue and U.S. Munonyedi,199054234253Kulkarni, M.P. and A.R. Juvekar,2009Alstonia scholaris (Linn.) R.Br. on stress and cognition in mice.]]>474752Kulkarni, S.K.,19993rd Edn.,pp: 133-134pp: 133-134Lakshmana, M.K. and T.R. Raju,1997246166170Lim-Sylianco, C.Y., A.P. Jocano and C.M. Linn,1990117231235Lin, S.C., C.C. Lin, Y.H. Lin, S. Supriyatna and S.L. Pan,1996Alstonia scholaris R. Br. on hepatotoxin-induced acute liver damage.]]>24153164Lucki, I.,19978523532Millan, M.J., A. Dekeyne, M. Papp, C.D. La Rochelle, C. MacSweeny, J.L. Peglion and M. Brocco,2001298581591Millan, M.J., M. Brocco, A. Gobert and A. Dekeyne,20052C receptor blockade.]]>177448458Nadkarni, A.K.,1976OECD Guidelines,20012001Pellow, S., P. Chopin, S.E. File and M. Briley,198514149167Prut, L. and C. Belzung,2003463333Rex, A., J.P. Voigt, C. Gustedt, S. Beckett and H. Fink,20041772334Saraswathi, V., N. Ramamoorthy, S. Subramaniam, V. Mathuram, P. Gunasekaran and S. Govindasamy,199844198205Saraswathi, V., V. Mathuram, S. Subramanian and S. Govindasamy,1999177988Silva, M.T.A., C.R.R. Alves and E.M.M. Santarem,199932333339Sonavane, G.S., V.P. Sarveiya, V.S. Kasture and S.B. Kasture,2002Myristica fragrans seeds.]]>71239244Takeuchi, H., S. Yatsugi, K. Hatanaka, K. Nakato and H. Hattori et al.,19972A receptor antagonistic activity.]]>3292735Tedeschi, R.E., D.H. Tedeschi, A. Mucha, L. Cook, P.A. Mattis, E.J. Fellows,19591252834Teste, J.F., I. Pelsy-Johann, T. Decelle and R.G. Boulu,19937219226Versha, P., B. Ghosh, B. Anroop and M. Ramanjit,2003Alstonia scholaris leaf extracts.]]>40412413