Microbiology Journal2153-06962153-0718Asian Network for Scientific Information10.3923/mj.2016.25.33Aspergillus flavus SWtS01 Through Random Mutagenesis]]>CyrusEkundayo Temitope JuwonArotupin Daniel 1-2201661-2Background and Objective: Aflatoxins production has limited the use of many strains of Aspergillus flavus in food fermentation and enzyme technology, despite their excellent abilities for copious enzyme production. In a bid to overcome this problem, the effects of some selected mutagenic agents on expression of aflatoxigenic traits in A. flavus was investigated in order to enhance its potential biotechnological uses. Materials and Methods: Spores of the strain were treated with chemical mutagens which include nitrous acid, sodium azide, ethyl methane sulphonate and hydroxylamine and ultraviolet radiation for 5400 sec. Mutants obtained at every 900 sec successively were stored under protection away from light for 24 h in order to stabilize DNA dimmers and the mutants were grown in aflatoxin production medium at 30°C for 10 days. Aflatoxins produced were quantified using enzyme-linked immunosorbent assay. Data obtained were subjected to analysis of variance using Statistical Package for Social Science (SPSS) version 17 at p≤0.05. Results: Ultraviolet treatment achieved 93.11% reduction of aflatoxins expression in the mutant CTUV75 (103.31±0.44 ppt) compared to wild strain. However, hydroxylamine showed 89.40% expression of aflatoxigenic traits in the CTNH2OH60 compared to the parent. Conclusion: Ultraviolet radiation caused considerable repression (93.11%) of aflatoxin production in the A. flavus strain in contrast to other mutagens. Nevertheless, the equivalent concentration of aflatoxin produced still exceeded 50 ppt limits set by FAO/WHO/EU. Thus, the use of such mutant in fermentation could pose public health risk. Therefore, it is suggested that further trials of UV treatment or other molecular methods may be employed whether aflatoxins production could be reduced below the standard limit or entirely get rid off in the mutant.]]>Ichishima, E.,2011Aspergillus Fungi used in Japanese Bioindustries.]]>Pages: 132Pages: 132Hoffmeister, D. and N.P. Keller,200724393416Varga, J. and R.A. Samson,2008Aspergillus in the Genomic Era.]]>Pages: 334Pages: 334Frisvad, J.C., C. Rank, K.F. Nielsen and T.O. Larsen,2009Aspergillus fumigatus.]]>475371Pitt, J.I. and A. Hocking,19992nd Edn.,pp: 375-383pp: 375-383Barnett, H.K. and B.B. Hunter,19723rd Edn.,Pages: 234Pages: 234Elliaiah, P., T. Prabhakar, B. Ramakrishna, A.T. Taleb and K. Adinarayana,2002Aspergillus niger for the production of lipase.]]>42151153Sharma, P.D.,19891st Edn.,pp: 33-35pp: 33-35Nadeem, M., J.I. Qazi and S. Baig,2010Bacillus licheniformis N-2 for dehairing.]]>5310151025Iftikhar, T., M. Niaz, S.Q. Abbas, M.A. Zia, I. Ashraf, K.J. Lee and Ikram-ul-Haq,2010Rhizopus oligosporus var. microsporus.]]>4212351249Rajeshkumar, J. and M.H.M. Ilyas,201112329Damisa, D., F.A. Kuta and N.U. Adabara,2013Aspergillus niger with hydroxylamine for improved cellulase synthesis from cellulosic wastes.]]>16572Irfan, M., J. Javed and Q. Syed,2011Aspergillus niger for enzyme production in submerged fermentation.]]>44137140Zaini, F., S. Yousefi, S. Dadgar and M. Safara,2009Aspergillus flavus isolates from green-tiger shrimps (Penaeus semisulcatus).]]>11822Cyrus, E.T. and A.D. Juwon,201510205213Hashida, M. and H. Bisgaard-Frantzen,200012389401Sambrook, J. and D.W. Russell,20013rd Edn.,Pages: 2344Pages: 2344Chang, P.K., J.W. Bennett and P.J. Cotty,2002Aspergillus parasiticus.]]>1534148Flaherty, J.E. and G.A. Payne,1997Aspergillus flavus.]]>6339954000Crawford, J.M., P.M. Thomas, J.R. Scheerer, A.L. Vagstad, N.L. Kelleher and C.A. Townsend,2008320243246Chang, P.K., J.W. Cary, D. Bhatnagar, T.E. Cleveland and J.W. Bennett et al.,1993Aspergillus parasiticus apa-2 gene associated with the regulation of aflatoxin biosynthesis.]]>5932733279Yu, J.,2012410241057Ichishima, E.,2007Aspergillus Fungus).]]>