Microbiology Journal2153-06962153-0718Asian Network for Scientific Information10.3923/mj.2015.43.48Acinetobacter baumannii ]]>DasmehHadis BaserisalehiMajid EmamiAmir 2201552Acinetobacter baumannii an antibiotic resistant bacterium was isolated and identified from clinical samples and assessed for plasmid carrying ability and the locations of resistant gene markers. A total of hundred and sixty six clinical samples were collected from the respiratory tracts and wound infections in the patients hospitalized in the Intensive Care Unit (ICU) of Namaze Hospital, Iran. The isolates were identified into phenotypes and genotypes and their antibiotic susceptibility assessed by disk diffusion method. The antibiotics used were: amikacin 30 μg, gentamicin 10 μg, imepenem 10 μg, meropenem 10 μg, ceftazidime 30 μg, ceftaxime 30 μg, ciprofloxacin 5 μg, polymyxin 300B and tetracycline 30 μg. The resistance strains of Acinetobacter baumannii was subjected to plasmid curing to find plasmid mediated resistance markers. The results showed that fifty one Acinetobacter spp. were isolated and all of them were identified as A. baumannii. In addition, the isolates were resistant to cefotaxime (100%) and gentamicin (90.1%) and susceptible to polymyxin (100%). The results from plasmid curing showed that the acridine orange could cure, the plasmids of A. baumannii cured. A. baumannii isolates were sensitive to ceftazidime, meropenem, cefotaxime, ceftazidine and amikacin. Therefore, antibiotic resistance markers associated with these antibiotics were plasmid encoded. The present study showed that A. baumannii is an important nosocomial infection in the ICU. Many antibiotic resistance markers in the A. baumannii were carried by the plasmids. Hence, they might be transmitted easily among the bacteria in the hospitals.]]>Antunes, L.C., F. Imperi, A. Carattoli and P. Visca,2011Acinetobacter baumannii pathogenicity.]]>2011Bauer, A.W., W.M.M. Kirby, J.C. Sherris and M. Turck,196645493496Beige, F., M.B. Salehi, N. Bahador and S. Mobasherzadeh,20152015Bergogne-Berezin, E.,2007Acinetobacter species as nosocomial pathogens.]]>3440444Brisson-Noel, A., P. Trieu-Cuot and P. Courvalin,1988221323Cambray, G., A.M. Guerout and D. Mazel,201044141166Chopade, B.A., P.J. Wise and K.J. Towner,1985Acinetobacter calcoaceticus EBF65/65.]]>13128052811Courvalin, P.,19943814471451Dale, J.W. and S. Park,20044th Edn.,Devaud, M., F.H. Kayser and B. Bachi,1982Acinetobacter strains.]]>22323329Dijkshoorn, L., A. Nemec and H. Seifert,2007Acinetobacter baumannii.]]>5939951Fournier, P.E., H. Richet and R.A. Weinstein,2006Acinetobacter baumannii in health care facilities.]]>42692699Gerner-Smidt, P.,1989Acinetobacter calcoaceticus.]]>142328Go, E.S., C. Urban, J. Burns, B. Kreiswirth and W. Eisner et al.,199434413291332Henwood, C.J., D.M. Livermore, D. James, M. Warner and Pseudomonas Study Group,2001Pseudomonas aeruginosa: Results of a UK survey and evaluation of the British Society for Antimicrobial Chemotherapy disc susceptibility test.]]>47789799Montefour, K., J. Frieden, S. Hurst, C. Helmich, D. Headley, M. Martin and D.A. Boyle,2008Acinetobacter baumannii: An emerging multidrug-resistant pathogen in critical care.]]>281525Murray, P.R. and E.J. Baron,20079th Edn.,pp: 133-151pp: 133-151Gordon, N.C. and D.W. Wareham,2010Acinetobacter baumannii: Mechanisms of virulence and resistance.]]>35219226Peleg, A.Y., H. Seifert and D.L. Paterson,2008Acinetobacter baumannii: Emergence of a successful pathogen.]]>21538582Song, W., K.M. Lee, H.J. Kang, D.H. Shin and D.K. Kim,200127136139Thomas, C.M. and K.M. Nielsen,20053711721