Journal of Pharmacology and Toxicology1816-496x2152-100xAcademic Journals Inc.10.3923/jpt.2020.8.15Bothrops asper from Different Localities]]>SarmientoKaren IvonneTorres RiosCarolina SalazarJulian BaracaldoAndrea ZambranoJorge Ramirez-ForeroGerman HernandezDiego PerezLuisa CastanoDiana DiezHugo 12020151Background and Objectives: Colombia does not produce fabotherapic antivenoms, the aim of current study was comparison of the neutralization of Bothrops asper venom from Atlantic and Andean region with 2 experimental monovalent antivenoms. Materials and Methods: The protein of the venom and antivenoms was quantified by absorbance at 280 nm and characterized by SDS-PAGE. The protein profile venoms were performed by RT-HPLC. It was calculated LD50 for the venoms and ED50 for the antivenoms, using Prism-statMate. An immunization scheme of 3 months was conducted in equine using Andean venom. The complete IgG antivenom was produced with caprylic acid, while the fabotherapic F(ab’)2 antivenom with pepsin. Both antivenoms were tested with Andean venom and Atlantic venom. Electrophoresis was conducted to verify the antivenoms by weight. Results: The protein was 11.5 mg/mL to Andean venom and 13.4 mg/mL to Atlantic venom. The SDS-PAGE showed bands of 15-20 kDa for both venoms. The LD50 to Andean venom was 2.8 and 2.3 mg/kg to Atlantic venom. The RT-HPLC showed similar protein fractions at first 40 min in both venoms. The protein for IgG was 9.8 mg/mL, while for F(ab’)2 it was 11.2 mg/mL. The SDS-PAGE showed bands of 130-200 kDa for IgG whyle of 94-150 kDa for F(ab’)2. The ED50 with Atlantic venom was 1.4 mg/mL using IgG and 1.75 mg/mL using F(ab’)2. The ED50 with Andean venom was 1.6 mg/mL using IgG and 1.9 mg/mL using F(ab’)2. Conclusion: Both antivenoms were efficient to neutralize the venom of Andean and Atlantic venom, without significant differences.]]>Instituto Nacional de Salud,20172017Instituto Nacional de Salud,20182018Rodríguez-Vargas, A.L., J. Rodriguez-Buitrago and G.J. Diaz,20121410011009Pérez, R.M.N.,2018Viperidae de diferentes regiones de Colombia mediante el empleo de cromatografía líquida y electroforesis.]]>2018Patiño, R.O., J.J.S. Haad, M.J.B. Acevedo, M.F.T. Castaño and J.C.Q. Castillo et al.,200720244262G-Biosciences,20152015Andrew, S.M. and J.A. Titus,2001212.8.12.8.10Pierce Chemical Technical Library,20152015De Roodt, A.R., S.I. García, C.M. Gómez, J. Estévez and A. Alagón et al.,2004122941Sanjuán-Galindez, J.A., J.A. Vargas, F.I. Ortíz, L.G. Gonzalez-Herrera, B. Watanabe-Minto and Y.T. Granja-Salcedo,2012Bothrops atrox en ratones albinos.]]>9147152Schroeder, Jr.H.W. and L. Cavacini,2010125S41S52WHO.,20162016Lomonte, B.,20072007Núñez, V., P. Cid, L. Sanz, P. De La Torres and Y. Angulo et al.,2009Bothrops atrox venoms from Colombia and the Amazon regions of Brazil, Perú and Ecuador suggest the occurrence of geographic variation of venom phenotype by a trend towards paedomorphism.]]>735778Scovino, S.,2017Bothrops, Bothrocophiasy Crotalus provenientes del valle del cauca Colombia (Fundación Zoológico de Cali) mediante el uso de cromatografía liquida de alta eficiencia HPLC y electroforesis.]]>2017Laemmli, U.K.,1970227680685De Roodt, A.R., S. Litwin, J. Estevez, E.G. Gould, J.A. Dolab and J. Gould,2010181020Rojas, G., J.M. Jimenez and J.M. Gutierrez,199432351363Jones, R.G.A. and J. Landon,2003275239250Pope, C.G.,193920132149Krifi, M.N., M. El Ayeb and K. Dellagi,19995128141Otero, R., V. Níñez, J. Barona, A. Díaz and M. Saldarriega,2002Bothrops asper y Porthidium nasutum de Antioquia y Chocó.]]>15515García, P.J., A. Yarlequé, C. Bonilla-Ferreyra, S. Pessah, D. Vivas, G.A. Sandoval and F. Lazo,2008Bothrops atrox.]]>25386390Rojas, E., L. Quesada, V. Arce, B. Lomonte, G. Rojas and J.M. Gutiérrez,2005Bothrops sp. snake venoms by polyvalent antivenoms produced in Perú and Costa Rica: Preclinical assessment.]]>938595Segura, A., M. Herrera, M. Villalta, M. Vargas, J.M. Gutiérrez and G. León,2013419397Gutierrez, J.M., G. Leon, B. Lomonte and Y. Angulo,201110369380Otero, R., G. León, J.M. Gutiérrez, G. Rojas and M.F. Toro et al.,2006Bothrops asper bites in Colombia.]]>10011731182Gutiérrez, J.M., G. León and B. Lomonte,200342721741