Journal of Pharmacology and Toxicology1816-496x2152-100xAcademic Journals Inc.10.3923/jpt.2019.18.27GhantaPratyusha BhatnagarUpendra PasumarthiSasidhar ChiliveriSunil Kumar 12019141Background and Objective: Cell based assays are simple tools with utilizing the conventional monolayer mode, but the absence of a real cell environment required an alternative technique to bridge the gap. This study was aimed to evaluate the possible application of three-dimensional cultures for cytotoxic testing. Materials and Methods: Three-dimensional (3D) cultures of murine fibroblasts or L-929 cells were grown by seeding a fixed density (1000 cells/well) in a 96 well plate coated with 1% w/v agarose. The spheroids were observed through different magnifications to observe its cellular structure. These cultures were treated against different types of toxicants and the reactivity was assessed qualitatively and quantitatively via XTT assay. Numerical data was calculated Kruskal Wallis-ANOVA followed by Dunnett post hoc for statistical differences. Results: Imaging of these fibroblastic spheroids at 1000 cells/ well density at different magnifications exhibited; compact spheroids, change in cell morphology and increased cell-to-cell interaction, indicating a similarity between the spheroid and an in vivo environment. Upon exposure with the zinc dibutyldithiocarbamate (ZDBC) powder, Sodium Lauryl Sulfate (SLS) and extract of ZDBC film and latex glove, the percentage cell viability was calculated to be directly proportional with the concentration of these compounds. Conclusion: The results confirmed the required sensitivity and specificity that can be used for biocompatibility testing.]]>Restle, L., D. Costa-Silva, E.S. Lourenco, R.F. Bachinski, A.C. Batista, A.B.R. Linhares and G.G. Alves,2015in vitro model for the evaluation of biomedical materials.]]>2015Chai, C. and K.W. Leong,200715467480ISO.,2009in vitro cytotoxicity.]]>2009USPC.,2016Pages: 458Pages: 458Ravi, M., V. Paramesh, S.R. Kaviya, E. Anuradha and F.P. Solomon,20152301626Lee, J., M.J. Cuddihy and N.A. Kotov,2008146186Antoni, D., H. Burckel, E. Josset and G. Noel,2015in vivo.]]>1655175527Wang, M.O., J.M. Etheridge, J.A. Thompson, C.E. Vorwald, D. Dean and J.P. Fisher,2013in vitro cytotoxicity of cross-linked biomaterials.]]>1413211329Duval, K., H. Grover, L.H. Han, Y. Mou, A.F. Pegoraro, J. Fredberg and Z. Chen,201732266277Mehta, G., A.Y. Hsiao, M. Ingram, G.D. Luker and S. Takayama,2012164192204Edmondson, R., J.J. Broglie, A.F. Adcock and L. Yang,201412207218Maritan, S.M., E.Y. Lian and L.M. Mulligan,20172017Li, W., J. Zhou and Y. Xu,2015in vitro cytotoxicity testing of medical devices.]]>3617620Zuckerbraun, H.L., H. Babich, R. May and M. Sinensky,1998in vitro. ]]>106628636Costa, E.C., V.M. Gaspar, P. Coutinho and I.J. Correia,201411116721685Ehrhart, F., J.C. Schulz, A. Katsen-Globa, S.G. Shirley and D. Reuter et al.,200958119127Fischer, E.R., B.T. Hansen, V. Nair, F.H. Hoyt and D.W. Dorward,20122222B.2.12B.2.47EN-ISO-10993-12,20082008Baek, H.S., J.Y. Yoo, D.K. Rah, D.W. Han, D.H. Lee, O.H. Kwon and J.C. Park,200546579583Sengün, A., M. Yalçın, H.E. Ülker, B. Öztürk and S.S. Hakkı,2011112e83e88Ulker, H.E. and A. Sengun,20093120126Idrees, A., V. Chiono, G. Ciardelli, S. Shah, R. Viebahn, X. Zhang and J. Salber,2018in vitro assays in three-dimensional human dermal constructs.]]>41779788Babich, H. and J.P. Babich,1997In vitro cytotoxicity studies with gingival cells.]]>91189196Fey, S.J. and K. Wrzesinski,2012127403411Lee, J., G.D. Lilly, R.C. Doty, P. Podsiadlo and N.A. Kotov,2009In vitro toxicity testing of nanoparticles in 3D cell culture.]]>512131221Sambale, F., A. Lavrentieva, F. Stahl, C. Blume and M. Stiesch et al.,2015205120129Fang, Y. and R.M. Eglen,201722456472