Journal of Pharmacology and Toxicology1816-496x2152-100xAcademic Journals Inc.10.3923/jpt.2012.46.51in vitro: Evidence Observed from Osmotic Fragility Test]]>AlnakshbandiAbdulkadir A. 1201271Cephalosporins adversely affect the hemopoietic system in terms of inducing immune hemolytic anemia and disturbing the function of blood platelets. In this study, the effect of 3rd generation cephalosporins, namely ceftriaxone, cefotaxime and ceftizoxime, on human red cells was investigated and the study was conducted in Rizgari Teaching Hospital in Hawler, Iraq. The effect of cephalosporins was evaluated by osmotic fragility test using fresh human blood in the presence of cephalosporins in varying concentrations between 25-200 μg mL-1. Although, all tested drugs shifted the osmotic fragility curve to some extent, the concentration of sodium chloride to induce 50% hemolysis (CH50) was higher in the presenceof ceftriaxone, cefotaxime and ceftizoxime at 200 μg mL-1 with increments in hemolysis percents of 5.55, 3.55, 3.16% in comparison to controls, respectively. As a result, this study shows that third generation cephalosporins at high concentrations alter directly the red cell membrane function in vitro, by shifting the osmotic fragility curve and increasing the levels of CH50.]]>Al-Hawsawi, Z.M., W.A. Turkistani, M.A. Al-Aidaros and D.L. Al-Harbi,20103826828Arndt, P.A. and G. Garratty,200542137144Baek, E.J, S. Lee, S. Kim, H.K. Choi and H.O. Kim,200929578584Davenport, R.D, W.J. Judd and L.R. Dake,200444849852Garratty, G.,200920097379Grossjohann, B., P. Eichler, A. Greinacher, S. Santoso and H. Kroll,20044410331040Johnson, S.T., J.T. Fueger and J.L. Gottschall,200747697702Imam, S.N., K. Wright, N. Bhoopalam and A. Choudhury,20089610611Leaf, D.E, N.B. Langer, M. Markowski, G. Garratty and D.L. Diuguid,2010124197199Montannez, M.I., C. Mayorga, M.J. Torres, A. Ariza, M. Blanca and E. Perez-Inestrosa,2011In vitro studies of chemical structure-IgE molecular recognition relationships.]]>24706717Moreira, L.L, T. Dias, L.G., Dias, M. Rogao, J.P. Da Silva and L.M. Estevinho,201149520526Quillen, K., C. Lane, E. Hu, S. Pelton and S. Bateman,200827357358Roll, E.B, T. Christensen and O.A. Gederaas,20059411431447Salama, A.,200987379Senturk, U.K, F. Gunduz, O. Kuru, G. Kocer and Y.G. Ozkaya et al.,20059914341441Sukoyan, G.V, M.R. Mumladze, E.D. Oboladze and N.A. Varazanashvili,2005In vitro effects of gentamicin, ampicillin and cefobid on energy supply and antioxidant protection systems of venous blood erythrocytes in newborns.]]>139671674Vani, R., C.S.S.S. Reddy and S.A. Devi,201054553562Tietz, N.W.,19953rd Edn.,Saha, S., R. Ramanathan, R.A. Basu, D. Banerjee and A. Chakrabarti,201187259266