Journal of Pharmacology and Toxicology1816-496x2152-100xAcademic Journals Inc.10.3923/jpt.2012.369.377BarlianAnggraini AnggadiredjaKusnandar PrihatinIndah 8201278Indonesia as tropical country is endemic to various infectious diseases, including that affecting the skin due to fungi infection. In nature, the eggshell, present as hard or soft shell, protects the embryo from microorganism infection. Turtle produces soft shells, which is very unique in that it does not contain albumin. In nature, the turtle eggshell has to cope with high humidity, so it is assumed that turtle soft shell has potent antifungal activity. This study investigated the potency of the eggshell extract from green sea turtle (Chelonia mydas) as an antifungal against Candida albicans, Trichophyton mentagrophytes, Microsporum gypseum and Aspergillus brasiliensis. Protein extraction was carried out with dialysis followed by lyophilization and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Antifungal activity assay was conducted by Dilution Plating method with nystatin as a standard drug. The possible mechanism of antifungal activity was assessed using Scanning Electron Microscopy (SEM). Fungal growth was inhibited by 8% w/v or 8x104 ppm extract, equivalent to 0.53 ppm nystatin against Candida albicans, 0.55 ppm against Microsporum gypseum, 0.36 ppm against Trichophyton mentagrophytes and 0.35 ppm against Aspergillus brasiliensis. The SDS-PAGE showed that the turtle eggshell extract had 6 proteins with molecular weight 20; 45, 50, 66, 80 and 116 kDa. This study showed that 8% w/v green turtle eggshell extract has inhibitory activity against Candida albicans, Trichophyton mentagrophytes, Microsporum gypseum and Aspergillus brasiliensis, as also proven by SEM results. Taken together, the present results showed that turtle eggshell extract has antifungal potency that could be developed as antifungal drug.]]>Adefemi, S.A., M.A. Abayomi and J.M. Abu,201029267270Ajibesin, K.K., N. Rene, D.N. Bala and U.A. Essiett,2008Allanblackia floribunda.]]>7129133Alam, S. and S. Mostahar,20055327333Andreoli, T.E. and M. Monahan,196852300325Andreoli, T.E., V.W. Dennis and A.M. Weigl,196953133156Barlian, A., K. Anggadiredja, A. Kusumorini and U. Ekawati,2011Duttaphrynus melanostictus frog skin and antifungal potency of the skin extract.]]>11196202Boman, H.G., I. Faye, V.P. Hofsten, K. Kockum, J.Y. Lee and K.G. Xanthopoulos,1985Hyalophora cecropina.]]>9551558De Lucca, A.J., J.M. Bland, C. Grimm, T.J. Jacks and J.W. Cary et al.,199844514520Conlon, J.M., E. Akhmed and E. Condamine,2009Acinetobacter baumannii.]]>74488493Conlon, J.M., E. Ahmed, T. Pal and A. Sonnevend,2010Acinetobacter baumannii.]]>3118061810De Lucca, A.J., T.J. Jacks and K.A. Brogden,1995151141148De Lucca, A.J., J.M. Bland, T.J. Jacks, C. Grimm, T.E. Cleveland and T.J. Walsh,199741481483De Lucca, A.J., J.M. Bland, T.J. Jacks, C. Grimm and T.J. Walsh,199836291298De Lucca, A.J.,20009273299Di Piro, J.T.,20032003pp: 358-370pp: 358-370Dulger, B. and A. Gonuz,20043104107Dulger, B. and A. Gonuz,2004715591562Hancock, R.E.,20011156164Havlickova, B., V.A. Czaika and M. Friedrich,200851215Horn, D.L., D. Neofytos, E.J. Anaissie, J.A. Fishman and W.J. Steinbach et al.,20094816951703Liu, Q. and C.L. Xiao,2005Potebniamyces pyri.]]>95572580Musyimi, D.M. and J.A. Ogur,2008Eucalyptus globules and Eucalyptus citriodora.]]>23543Pfaller, M.A. and W.L. Yu,20011512271261Pieme, C.A., J.P. Dzoyem, F.A. Kechia, F.X. Etoa and V. Penlap,2008In vitro antimicrobial activity of extracts from some Cameroonian medicinal plants.]]>8902907Simmaco, M., G. Mignogna, D. Barra and F. Bossa,1993Rana esculenta.]]>324159161Simmaco, M., G. Mignogna, S. Canofeni, R. Miele, M.L. Mangoni and D. Barra,1996Rana temporaria.]]>242788792Sittiwet, C., D. Puangpronpitag and N. Niamsa,2009In vitro antimicrobial activity of Schefflera leucantha: The potential of respiratory tract and urinary tract infection treatment.]]>5240243Subasinghage, A.P., J.M. Conlon and C.M. Hewage,2010Silurana tropicalis: Conformational analysis of the amphipathic, alpha-helical antimicrobial peptide XT-7 and its non-haemolytic analogue [G4K]XT-7.]]>180410201028Sunilson, J.A.J., R. Suraj, G. Rejitha, K. Anandarajagopal, A.V.A.G. Kumari and P. Promwichit,2009In vitro antimicrobial evaluation of Zingiber officinale, Curcuma longa and Alpinia galanga extracts as natural food preservatives.]]>4192200Theis, T. and U. Stahl,200461437455Lakshminarayanan, R., S. Vivekanandan, R.P. Samy, Y. Banerjee and E.O. Chi-Jin et al.,200813046604668Wellman-Labadie, O., J. Picman and M.T. Hincke,200849133143Wellman-Labadie O., S. Lemaire, K. Mann, J. Picman and M.T. Hincke,2010581015610161