Journal of Pharmacology and Toxicology1816-496x2152-100xAcademic Journals Inc.10.3923/jpt.2011.272.282Boesenbergia Species on the Proliferation of Several Cancer Cell Lines]]>JingLing Jing Abu BakarMohd Fadzelly MohamedMaryati RahmatAsmah 3201163The aim of this research was to determine the potential of Boesenbergia species collected from Sabah rainforest as anticancer remedy. This research was done to Boesenbergia rotunda, B. pulchella, B. pulchella var attenuata and B. armeniaca crude extracts on the in vitro proliferation of hormone-dependent breast cancer (MCF-7), non-hormone dependent breast cancer (MDA-MB-231), ovarian cancer (CaOV3), colon cancer (HT-29) and cervix cancer (Hela) cell lines. The effects of the four Boesenbergia species (methanol extracts of leaves, stem and rhizome part) on the proliferation of MCF-7, MDA-MB-231, CaOV3, HT-29 and Hela cell lines were measured using (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay. The crude extracts with IC50 value less than 100 μg mL-1 were subjected to cell cycle analysis by using flow cytometry for DNA content determination. B. rotunda as positive control showed significant inhibitions towards all the cancer cell lines tested with IC50 ranging from 51.0±0.01 to 71.0±1.41 μg mL-1. The remaining Boesenbergia species only showed positive cytotoxicity activities against MCF-7 cell lines especially B. pulchella var attenuata with IC50 value 93.0±2.83 μg mL-1 and B. armeniaca with 94.5±0.71 μg mL-1. In cell cycle analysis, B. rotunda crude extract arrested cell at sub-G1 phase while B. pulchella var attenuata arrested cell at G2/M phase. From this study, it can be concluded that only B. pulchella var attenuata and B. armeniaca were effective against cellular proliferations of MCF-7. However, both species were not as effective as B. rotunda.]]>Abu Bakar, M.F., M. Mohamed, A. Rahmat and J. Fry,2009Mangifera pajang) and tarap (Artocarpus odoratissimus).]]>113479483Abu Bakar, M.F., M. Mohamed, A. Rahmat, S.A. Burr and J.R. Fry,2010Mangifera pajang and Artocarpus odoratissimus fruits.]]>402938Burkill, I.H.,1935Vol. I,pp: 1078-1079pp: 1078-1079Chan, E.W.C., Y.Y. Lim, L.F. Wong, F.S. Lianto and S.K. Wong et al.,2008109477483Censini, S., C. Lange, Z. Xiang, J.E. Crabtree and P. Ghiara et al.,1996Helicobacter pylori, encodes type I-specific and disease associated virulence factors.]]>931464814653Frankel, S. and M. Berenbaum,199931422429Hasnah, M.S., J. Shajarahtunnur and M. Neelavany,1995Boesenbergia species.]]>1995Heyne, K.,1987pp: 592-594pp: 592-594Herrmann, K.,198818615Huang, Y.T., J.J. Hwang, P.P. Lee, F.C. Ke and J.H. Huang et al.,19991289991010Kirana, C., G.P. Jones, I.R. Record and G.H. McIntosh,2007Boesenbergia pandurata (Zingiberaceae).]]>61131137Khaw, S.H.,2001Etlingera (Zingiberaceae) in peninsular Malaysia including a new species.]]>53191239Larsen, K.,20032003pp: 15Ling, J.J., M. Mohamed, A. Rahmat and M.F. Abu Bakar,2010Boesenbergia rotunda, Boesenbergia pulchella var. attenuata and Boesenbergia armeniaca).]]>42732Ling, J.J.,2010Boesenbergia species (Zingiberaceae) endemic to borneo.]]>2010Mahady, G.B.,20051124052427Masuda, T. , S. Yonemori, Y. Oyama, Y. Takeda and T. Tanaka,19994717491754Mosmann, T.,1983655563Schwartsmann, G., M.J. Ratain, G.M. Cragg, J.E. Wong and N. Saijo et al.,2002204759Seeff, L.B., K.L. Lindsay, B.R. Bacon, T.F. Kresina and J.H. Hoofnaqle,200134595603Su, T.L. and K.A. Watanabe,1993Vol. 13,Sun, T. and C.T. Ho,200590743749Kiat, T.S., R. Pippen, R. Yusof, H. Ibrahim, N. Khalid and N. Abd Rahman,2006Boesenbergia rotunda (L.), towards dengue-2 virus NS3 protease.]]>1633373340Thaipong, K., U. Boonprakob, K. Crosby, L. Cisneros-Zevallos and D.H. Byrne,200619669675Tewtrakul, S., S. Subhadhirasakul, J. Puripattanavong and T. Panphadung,2003Boesenbergia pandurata Holtt.]]>25503508Wei, Y.Q., X. Zhao, Y. Kariya, H. Fukata, K. Teshigawara and A. Uchida,19945449524957Wettasinghe, M., B. Bolling, P. Plhak and K. Parkin,20026725832588Winslow, L.C. and D.J. Kroll,199815821922199Yuan, S.I., Y.Q. Wei, X.J. Wang, F. Xiao, S.F. Li and J. Zhang,20041020242028Yun, J.M., H. Kwon and J.K. Hwang,2003In vitro anti-inflammatory activity of panduratin A isolated from Kaempferia pandurata in RAW264.7 cells.]]>6911021108