Journal of Pharmacology and Toxicology1816-496x2152-100xAcademic Journals Inc.10.3923/jpt.2006.270.277PrasanthiR.P. Jaya ReddyG. Hariprasad ChettyC.S. ReddyG. Rajarami 3200613Developing nervous system has long been recognized as a primary target for lead (Pb2+) induced toxicity. We have examined the effect of calcium (Ca2+) and zinc (Zn2+) supplementation on Pb2+-induced alterations in adenosine triphosphatase (ATPase) activities in the developing mouse brain from postnatal day (PND) 14 to 90. Albino mice were lactationally exposed to low (0.2%) and high (1%) levels of lead acetate via the drinking water of the mother. Pb2+-exposure was commenced on PND 1, continued up to PND 21 and stopped at weaning. Ca2+ and Zn2+ (0.02% in 0.2% Pb2+-water or 0.1% in 1% Pb2+-water) were supplemented separately to the mother up to PND 21. The activities of Mg2+ATPase in mitochondrial fraction and Na+K+ATPase in P2-fraction of the cerebral cortex, hippocampus, cerebellum and medulla were assayed. The specific activity of both ATPases increased gradually in an age-dependent manner in the control brain. Pb2+-exposure significantly inhibited the activities of both Mg2+ATPase and Na+K+ATPase in different brain regions of the developing brain. The inhibition was more pronounced at PND 28 in the brain regions of mice exposed to 1% Pb2+. Ca2+ or Zn2+-supplementation significantly reversed the Pb2+-inhibited activity of both ATPases. These results indicate that dietary Ca2+/Zn2+-supplementation decreases intestinal absorption of Pb2+ and there by reduces the neurotoxic effects of Pb2+.]]>Barcellos, C.K., M.R.C. Schetinger, A.M.O. Battastini, L.B. Silva, R.D. Dias and J.J.F. Sarkis, 19942711111115Bertoni, J.M. and P.M. Sprenkle, 19914821492156Bettaiya, R., P.R. Yallapragada, E. Hall and S. Rajanna, 1996 In vitro effect of lead on Ca2+-ATPase in synaptic plasma membranes and microsomes of cerebral cortex and cerebellum. ]]>33157162Bhattacharya, S., S. Bose, B. Mukhapadhyay, D. Sarkar and D. Das et al .,1997+K+-ATPase in rat liver plasma membrane and signal transduction.]]>10157162Bogden, J.D., S.B. Gertner, S. Christakos, F.W. Kemp, Z. Yang, S.R. Katz and C. Chu, 199212213511360Bradbury, M.W. and R. Deane, 199314131136Bressler, J.P. and G.W. Goldstein, 199141479484Cardone, E., M.A. Lessler and G.P. Brierley,1971136300312Carfagna, M.A., G.D. Ponsler and B.B. Muhoberac, 19961005365Cerklewski, F.L. and R.M. Forbes, 1976106689696Chetty, C.S., B. Rajanna and S. Rajanna, 1990+K+-ATPase and ouabain binding by mercuric chloride. ]]>51109116Cotman, C.W. and D.A. Matthews,1971249380394Devi, C., G. Reddy and C. Chetty,200523375381Fiske, C.H. and Y. Subbarow,192566375400Flora, S.J.S. and S.K. Tandon,199064129139Fox, D.A., S.D. Rubinstein and P. Hsu, 1991+ K+ATPase. ]]>109482493Hammond, P.B., R.L. Bornschein, H. Zenick,198455366Jablonska, L., M. Walski and U. Rafalowska,199414701709Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Luthman, J., E. Lindqvist, G.A. Gerhardt, L. Olson and B.H. Hoffer,199465100118Moreira, E.G., I. Vassilieff and V.S. Vassilieff,200123489495Peraza, M.A., F. Fierro, D.S. Barber, E. Casarez and L.T. Rael, 1998106203216Pires, J.B., N. Miekeley and C.M. Donangelo,2002175247255Prasanthi, R.P.J., G.H. Reddy, C.B. Devi and G.R. Reddy, 200518615626Reddy, G.R., M.R. Basha, C.B. Devi, A. Suresh and J.L. Baker et al.,200321347352Rohn, T.T., T.R. Hinds and F.F. Vincenzi, 19932+ pump ATPase and Na+/K+ pump ATPase of human red blood cell membranes.]]>46525534Sagara, Y., R. Dargusch, F.G. Klier, D. Schubert and C. Behl,199616497505Schmidt-Niensen, B.,19744623012315Siegel, G.J., S.K. Fogt and M.J. Hurley, 19771977pp: 465-493pp: 465-493Struzynska, L., B. Dabrowska-Bouta, J. Lenart, J. Zborowski and U. Rafalowska, 1994in vivo and in vitro.]]>425562Sushma, N.J., 1999Sweadner, K.J. and S.M. Goldin,1980302777783Tirri, R., K.Y.H. Lagerspetz and J. Kohnen, 197344B473481Tucker, R.K. and A. Matte, 1980In vitro effects of cadmium and lead on ATPases in the gill of rock crab Cancer teroratus.]]>24847852Valle, B.L. and D.D. Ulmer, 1972419199Widzowski, D.V. and D.A. Cory-Slechta, 199415295307Winder, C. and I. Kitchen, 1984225987