Journal of Plant Sciences1816-4951xxxx-xxxxxAcademic Journals Inc.10.3923/jps.2016.61.68Tectona grandis Linn f.)]]>MustariEri DiningratDiky Setya RatnasihRina WidiyantoSrinanan M. 42016114Background: Teak (Tectona grandis Linn f.) is one of valuable timber species because of the beauty, strength and durability properties. Main problem in teak improvement program is reproductive biology, a low pollen viability, high embryo abortion, low pollination success and low fruit production. Investigation carried out at the teak improvement that flowering aspects is very important. Teak development program was facing difficulties due to the lack of information about the role of genes regulating flowering. APETALA2 (AP2) and APETALA3 (AP3) genes as member of the floral organ Identity. The role of teak flowering development predicted by up and down regulation of TgAP2 and TgAP3 genes. This study was conducted to determine the gene expression profile of TgAP2 and TgAP3 in the developmental stages of teak flowering. Materials and Methods: In this study, TgAP2 and TgAP3 genes in teak identified from NGS transcriptome data that is annotated with Solanum lycopersicum. The TgAP2 and TgAP3 genes activities on the several developmental stages of teak flowering tissues are done with QRT-PCR analysis using 18S RNA as gene reference. Results: The highest expression level of TgAP2 on the floral development stages of 4th lateral floral buds. The TgAP3 gene expression reached the highest level on the apical floral bud stage development. Vegetative shoot as control stages, TgAP2 gene expression level reached 9 times compared to the control and TgAP3 more than 12 times. The TgAP2 and TgAP3 genes expression profile has non-significant correlation on all floral development stages. Conclusion: The TgAP2 and TgAP3 genes expressed with different level in all stages of the teak flowering development. The TgAP2 and TgAP3 genes have non-significant correlation expression profile in role of teak floral development. These results indicate that the TgAP2 and TgAP3 expression profiles equivalent to the general pattern in the Arabidopsis model plant.]]>Alcantara, B.K. and E.A. Veasey,2013Tectona grandis L.F.) from different provenances using microsatellite markers.]]>37747758Norlia, B., M. Norwati, A. Norwati, H.M. Rosli and M.S. Norihan,2008Tectona grandis (teak).]]>713021308Blazquez, M.A.,200011335473548Bowman, J.L., D.R. Smyth and E.M. Meyerowitz,1991Arabidopsis.]]>112120Coen, E.S. and E.M. Meyerowitz,19913533137Coen, E.S. and R. Carpenter,1993511751181Cseke, L.J. and G.K. Podila,200410728Diningrat, D.S., S.M. Widiyanto, A. Pancoro, Iriawati, B. Panchangam and J.E. Carlson,2013Tectona grandis Linn f.).]]>2013Diningrat, D.S., S.M. Widiyanto, A. Pancoro, A. Iriawati and D. Shim et al.,2015Tectona grandis, L.F) in vegetative to generative stages development.]]>10114Diningrat, D.S., S.M. Widiyanto, A. Pancoro, Iriawati and D. Shim et al.,2015Tectona grandis) floral development regulation using RNA-seq.]]>10113Ferrario, S., R.G.H. Immink and G.C. Angenent,200478491Howe, G.T., J. Yu, B. Knaus, R. Cronn and S. Kolpak et al.,2013De novo transcriptome assembly and SNP detection and validation.]]>2013Jack, T.,200416S1S17Jian, B., B. Liu, Y. Bi, W. Hou, C. Wu and T. Han,20082008Kokutse, A.D., A. Stokes, H. Bailleres, K. Kokou and C. Baudasse,2006Tectona grandis L.F) heartwood and relationship with colour.]]>20219223Koressaar, T. and M. Remm,20072312891291Krizek, B.A. and J.C. Fletcher,20056688698Liu, T., S. Zhu, Q. Tang, P. Chen, Y. Yu and S. Tang,2013De novo assembly and characterization of transcriptome using illumina paired-end sequencing and identification of CesA gene in ramie (Boehmeria nivea L. Gaud).]]>2013Livak, K.J. and T.D. Schmittgen,2001-ΔΔCT method.]]>25402408Park, J.H., Y. Ishikawa, T. Ochiai, A. Kanno and T. Kameya,2004GLOBOSA-like genes are expressed in second and third whorls of homochlamydeous flowers in Asparagus officinalis L.]]>45325332Palupi, E.R., J.N. Owens, S. Sadjad, S. Sudarsono and D.D. Solihin,2010Tectona grandis).]]>4022042214Putterill, J., R. Laurie and R. Macknight,200426363373Trindade, I., C. Capitao, T. Dalmay, M.P. Fevereiro and D.M. dos Santos,2010Medicago truncatula.]]>231705716Untergasser A., I. Cutcutache, T. Koressaar, J. Ye, B.C. Faircloth, M. Remm and S.G. Rozen,20122012Wellmer, F., M. Alves-Ferreira, A. Dubois, J.L. Riechmann and E.M. Meyerowitz,20062006Widiyanto, S.N., A. Pancoro, A.M. Brunner and S.H. Strauss,2009Tectona grandis L.F.).]]>2009pp: 4148Zahn, L.M., J.H. Leebens-Mack, J.M. Arrington, Y. Hua and L.L. Landherr et al.,2006AGAMOUS subfamily of MADS-box genes: Evidence of independent sub- and neofunctionalization events.]]>83045