Journal of Plant Sciences1816-4951xxxx-xxxxxAcademic Journals Inc.10.3923/jps.2016.38.44Phytophthora sp. Causing Root Rot of Cassava and Plant Growth Promoting Activities]]>KhucharoenphaisanK. RodbangpongK. SaengpaenP. SinmaK. 1-32016111-3Actinomycetes have been promised as biocontrol and stimulating agents for use in agriculture without detrimental effects to the environmental due to their antifungal with secondary metabolites produced. The aim of this study was to screen soil actinomycetes according to its ability to produce various secondary metabolites against Phytophthora sp. that causing root rot disease of cassava and stimulating agent has also determined. Firstly, soil actinomycetes were isolated and tested for antagonistic activity toward the fungus by the dual culture technique. After that the selected isolate was determined on the stimulating agent as IAA production. Finally, extracellular anti-fungal metabolites produced by selected isolates were evaluated for anti-fungal potential toward the fungus with agar core technique. The result showed that 98 isolates from soil samples were screened on their anti-fungal activity. Among these, 38 isolates showed the inhibition activities against Phytophthora sp. in which was isolated from infected cassava. The culture supernatants without cell obtained from 16 isolates were affective against the fungus whereas 10 isolates produced affective thermostable compound. In total, the isolate LB35 was most promising on the basis of its interesting antimicrobial activity and could produce IAA with 50 μg mL1. Based on its 16S rDNA sequence and phylogenetic tree analysis, isolate LB35 belong to the Streptomyces malaysiensis. The addition of isolate LB35 as fresh inoculums to the cassava field, the cassava showed more height comparing to control experiment. Moreover, S. malaysiensis has not to be phytopathogenic microorganism of cassava. This finding has been increased scope of agriculturally important actinomycetes applications.]]>Adeoti, O.,201034985992Aghighi, S., G.H.S. Bonjar, R. Rawashdeh, S. Batayneh and I. Saadoun,2004Alternaria solani, Alternaria alternate, Fusarium solani, Phytophthora megasperma, Verticillium dahliae and Saccharomyces cerevisiae.]]>3463471Barros, J.A., E.V. Medeiros, K.A. Notaro, W.S. Moraes, J.M. Silva, T.C.E.S. Nascimento and K.A. Moreira,2014Fusarium solani.]]>8967973Bennett, M.J., A. Marchant, S.T. May and R. Swarup,199835315111515Bloemberg, G.V. and B.J.J. Lugtenberg,20014343350Boonlue, S., T. Aimi and T. Morinaga,200347119124Buensanteai, N. and D. Athinuwat,2012Trichoderma virens strain TvSUT10 against cassava stem rot in Thailand.]]>111499615001Chaisinboon, O. and I. Chontanawat,20119216229Cheng, J., S.H. Yang, S.A. Palaniyandi, J.S. Han, T. Yoon, T.J. Kim and J. Suh,2010Streptomyces malaysiensis MJM1968 isolated from agricultural soil.]]>53545552Cuesta, G., R. Garcia-de-la-Fuente, M. Abad and F. Fornes,201295S280S284Dobbelaere, S., A. Croonenborghs, A. Thys, A. Vande Broek and J. Vanderleyden,1999Azospirillum brasilense wild type and mutant strains altered in IAA production on wheat.]]>212153162Doumbou, C.L., M.K. Hamby-Salove, D.L. Crawford and C. Beaulieu,20018285102Elamvazhuthi, P. and M. Subramanian,201326672Felsenstein, J.,198539783791Fukuda, C.,19911991pp: 1-2pp: 1-2Gangwar, M., S. Rani and N. Sharma,201231523Goudjal, Y., O. Toumatia, N. Sabaou, M. Barakate, F. Mathieu and A. Zitouni,20132918211829Hayakawa, M. and H. Nonomura,198765501509Hirscha, A.M. and M. Valdesc,2010Micromonospora: An important microbe for biomedicine and potentially for biocontrol and biofuels.]]>42536542Khamna, S., A. Yokota and S. Lumyong,200925649655Khucharoenphaisan, K., U. Puangpetch, K. Puttaraksa and K. Sinma,201111314319Khucharoenphaisan, K. and K. Sinma,2011Saccharopolyspora sp. PNR11.]]>14226231Khucharoenphaisan, K., N. Sripairoj and K. Sinma,201276873Khucharoenphaisan, K., K. Sinma and C. Lorrungruang,201313472478Khucharoenphaisan, K., C. Suinongpai and K. Sinma,20142014Li, W., M. Csukai, A. Corran, P. Crowley, P.S. Solomon and R.P. Oliver,2008Stagonospora nodorum (Berk) Castell and Germano, the cause of wheat glume blotch disease.]]>6412941302Nonoh, J.O., W. Lwande, D. Masiga, R. Herrmann and J.K. Presnail et al.,2010Streptomyces species with antifungal activity from selected national parks in Kenya.]]>4856864Peciorek, T. and J. Friml,200611911991202Prapagdee, B., C. Kuekulvong and S. Mongkolsuk,2008Streptomyces hygroscopicus against phytopathogenic fungi.]]>4330337Sharma, S.B., R.Z. Sayyed, M.H. Trivedi and T.A. Gobi,20132013Shirling, E.B. and D. Gottlieb,1966Streptomyces species.]]>16313340Sinha, K., R. Hegde and A. Kush,201433745Suwan, N., W. Boonying and S. Nalumpang,2012Colletotrichum gloeosporioides.]]>8725737Taechowisan, T., N. Chuaychot, S. Chanaphat, A. Wanbanjob and P. Tantiwachwutikul,2009Streptomyces sp. SRM1 on Colletotrichum musae.]]>88692