Journal of Plant Sciences1816-4951xxxx-xxxxxAcademic Journals Inc.10.3923/jps.2013.57.64Vatica diospyroides Symington,
a Critically Endangered Medicinal and Fragrant Plant in the Dipterocarpaceae]]>SrisawatTheera KanjanasopaDuangkhaetita IeamkhengSupaporn Cheur-SrisakulRommanee 2201382Vatica diospyroides Symington has two distinct commonly known subtypes:
The SS and LS forms distinguished by the color of petals and cotyledons. These
subtypes may also differ in their contents of the valuable products for human
health-therefore it is important to develop and share a reliable method of subtype
identification. The Random Amplified Polymorphic DNA (RAPD) technique was applied
with 3x and 5xCTAB (Cetyltrimethyl ammonium bromide) extraction procedures,
the concentrations of NaCl and DNA templates were varied and 10-mer random woody
primers were screened to find distinguishing genetic indicators. The best yields
of SS and LS DNA (412.8-578.4 μg g-1 FW) were found with phenol:
Chloroform: Isoamyl alcohol (25:24:1) followed by 5xCTAB buffer. Five effective
primers (OPU5, OPU8, OPU9, OPU14 and OPU15) gave a total of 18 DNA bands (200-2500
bp), when using 1.5 mM NaCl and 200 ng μL-1 DNA template in
PCR conditions. Of the bands, 12 were evidently polymorphic. The primer OPU9
produced 1 polymorphic band (220 bp) for LS and 1 monomorphism (at 500 bp).
With OPU15, a total of 4 bands were observed: The markers at 320, 480 and 520
bp specify the LS form, while at 900 bp the band is shared by both subtypes.
In conclusion, the V. diospyroides subtypes distinguished by different
color petals and cotyledons, are also genetically differentiated: The phenotypic
differences are not environmentally induced. These initial results encourage
pursuing genomics based phylogenetic analyses of V. diospyroides.]]>Ashton, P.,19981998Atun, S., N. Aznam, R. Arianingrum, Y. Takaya and N. Masatake,2008Hopea and their biological activity test.]]>19721Bock, R.,19971997Campey, M.L., M. Waycott and G.A. Kendrick,2000Posidonia ostenfeldii species complex (Posidoniaceae)-morphological and genetic variation.]]>664156Doyle, J.J.,1990121315Ito, T., Y. Akao, H. Yi, K. Ohguchi and K. Matsumoto et al.,20032414891497Kaewmuangmoon, J., T. Suwanvijitr, W. Cherdshewasart and C. Chanchao,201036180186Panichayupakaranant, P., C. Kortanakul and P. Rattanasuwan,2006Aquilaria sp.).]]>2006Pooma, R.,200230727Pooma, R. and M. Newman,200129110187Li, Q.M., Z.F. Xu and T.H. He,2002Vatica guangxiensis (Dipterocarpaceae).]]>44246249Rajaseger, G., H.T.W. Tan, I.M. Turner and P.P. Kumar,199780355361Rath, P., G. Rajaseger, C.J. Goh and P.P. Kumar,1998826165Sathish, D.K. and C. Mohankumar,2007Elaeis guineensis Jacq.) parental varieties (dura & pisifera) and the hybrid tenera.]]>6354358Seo, E.K., H. Chai, H.L. Constant, T. Santisuk and V. Reutrakul et al.,1999Vatica diospyroides.]]>6469766983Sheidai, M., Z. Noormohammadi, A.R. Dehghani, F. Parvini, H. Hoshiar-Parsian and M. Hosseini-Mazinani,2010Olea cuspidata Wall ex. DC.) of Iran.]]>36187193Smitinand, T.,19661966pp: 6776Srinual, A. and A. Thammathaworn,2008Vatica L. (Dipterocarpaceae) in Thailand.]]>8121134Srisawat, T., K. Kanchanapoom, K. Pattanapanyasat, S. Srikul and W. Chuthammathat,200527645652Srisawat, T., S. Thipnetr and C. Maknoi,2012Vatica diospyroides Symington, endangered medicinal plant of Peninsular Thailand.]]>636813688Wilson, T.D., R.M. Brook and H.F. Tomlinson,1998Parkia biglobosa) and under-planted sorghum in a parkland system in Burkina Faso.]]>348598Young, N.D.,20002000pp: 31-47pp: 31-47Zain, W.Z.W.M., N. Ahmat, N.H. Norizan and N.A.A.M. Nazri,20115926929