Journal of Plant Sciences1816-4951xxxx-xxxxxAcademic Journals Inc.10.3923/jps.2007.504.517Agrobacterium-mediated Transformation with a Rice Chitinase Gene]]>MaziahM. SreeramananS. PuadA. SariahM. 5200725A rice chitinase gene (RCC2) multiplied in Agrobacterium strain (EHA 101), was simultaneously introduced into single buds of in vitro grown banana cultivar, Rastali (AAB). Plasmid pBI333-EN4-RCC2 contained a hygromycin phosphotransferase gene (hpt11) as the selectable marker to identify the transformants. Treatment A contained hygromycin at 25 mg L-1 and treatment B contained hygromycin at 50 mg L-1 in both MS medium supplemented 5 mg L-1 of BAP together with 2.7 g of gelrite agar. Single buds derived from multiple bud clumps (Mbcs), were the target explants for transformation. An assay was performed to identify the minimum concentration required for two antibiotics (carbenicillin and cefotaxime) that is most effective against Agrobacterium strain, EHA 101 and the effect on tissue regeneration capacity. Even though the transformation frequency based on hygromycin selection medium (treatment A) is higher, but there is no transformant could be confirmed based on PCR and Southern blot analyses, as compared using 50 mg L-1 hygromycin selection medium. Assay of protein extract from the transgenic plantlets showed an increased in chitinase enzyme activity over the untransformed plantlets. The present of Agrobacterium-mediated transformation reported here is suitable for using tiny meristem tissues to obtain fungal disease tolerant or resistant banana through genetic engineering.]]>Aldemita, R.R. and T.K. Hodges,1996Agrobacterium tumefaciens-mediated transformation of japonica and indica rice varieties.]]>199612617Anfoka, G. and H. 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