Journal of Plant Sciences1816-4951xxxx-xxxxxAcademic Journals Inc.10.3923/jps.2007.407.415Solanum melongena L.)]]>Hossa inM. J.RahmanM. BariM. A.4200724The aim of this study to show, an efficient protocol for establishment of cell suspension culture and plantlet regeneration through cell culture from the cotyledonary explants of Brinjal (Solanum melongena L.). In this investigation, three varieties of Brinjal cv. Loda, China and Jhotika were used. In first step, the somatic embryogenic calli formation was done using MS medium supplemented with different concentrations of auxin and cytokinin singly or in combination. Cells of the three varieties were isolated from the rapidly growing embryogenic and friable calli using orbital shaker. For callus induction the isolated cells were transferred to MS liquid medium containing different hormonal concentrations and after 37-63 days of incubation the micro-calli were appeared. The Loda and China varieties showed the best result (8.0 and 8.2%, respectively) in 2 mg L-1 NAA+0.05 mg L-1 BAP and 2 mg L-1 2,4-D+0.05 mg L-1 BAP. For embryo formation, micro-calli were subcultured on MS solid medium and the Loda variety showed the best result (21%) in the medium containing 1.0 mg L-1 BAP+0.05 mg L-1 GA3. The bipolar embryos were selected and cultured in MS medium with different combinations and concentrations of auxin (NAA) and cytokinin (BAP and IBA) for shoot and root formation. Optimum shoot and root formations were recorded in MS medium supplemented with 0.75 mg L-1 NAA+1.5 mg L-1 BAP and 2.0 mg L-1 NAA+0.5 mg L-1 IBA, respectively. The plantlets appeared in the embryo mass were cultured and acclimatized.]]>Anisuzzaman, M., A.H.M. Kamal, R. Islam, M. Hossain and O.I. Joarder,1993Solanum melongena L.]]>33540Das, G.P., S. Ramaswamy and M.A. Bari,20002000Daunay, M.C., R.N. Lester and H. Laterrot,1991Solanum melongena L.) and Tomato (Lycopersicon esculentum).]]>1991pp: 389-413pp: 389-413Fari, M., I. Nagy, M. Csanyi, J. Mityko and A. Andrasfalvy,1995Agrobacterium mediated enetic transformation and plant regeneration via organogenesis and somatic embryogenesis from cotyledon leaves in eggplant (Solanum melongena L. cv. Kecskemeti Lela).]]>158286Franklin, G. and G.L. Sita, 2003Solanum melongena L.) using root explants.]]>21549554Fassuliotis, G., B.V. Nelson and D.P. Bhatt,1981Solanum melongena cv. Florida market.]]>22119125Gleddie, S., W.A. Keller and G. Setterfield,1983Solanum melongena (eggplant).]]>61656666Guri, A. and K.C. Sink,1988Agrobacterium transformation of eggplant.]]>1335255Huda, A.K.M.N. and B. Sikdar,20032003Kamat, M.G. and P.S. Rao,1978Solanum melongena) using tissue culture techniques.]]>135765Magioli, C., A.P.M. Rocha, D.E. de Oliveira and E. Mansur,1998Solanum melongena L.) induced by thidiazuron.]]>17661663Matsuoka, H. and K. Hinata,1979Solarium melongena L.]]>30363370Murashige, T. and F. Skoog,196215473497Nasir, M.,20022002Rao, N.N.,1979Solanum melongena and Some Other Species of Solanum.]]>1979Sadilova, E., F.C. Stintzing, R. Carle, J. Van Eck and A. Snyder,2006Solanum melongena L.) and violet pepper (Capsicum annuum L.).]]>343439447Sarker, R.H., S. Yesmin and M.I. Hoque,2006Solanum melongena L.).]]>165361Sarma, P. and M.V. Rajam,1995Solanum melongena L.).]]>46135141Taha, R.M. and M. Tijan,2002in vitro production and field transfer protocol for Solanum melongena plants.]]>68447450Yadav, J.S. and M.V. Rajam,1998116617625