Journal of Plant Sciences1816-4951xxxx-xxxxxAcademic Journals Inc.10.3923/jps.2007.273.282YaoXiao ZhangJianxia ChenGang 3200723Capillary electrophoresis with electrochemical detection has been employed for the determination of three phenolic acetophenones (including paeonol, p-hydroxyacetophenone and 2,4-hydroxyacetophenone) in Radix Cynanchi Paniculati (i.e., Paniculate Swallowworf Root) for the first time. Effects of several important factors such as the concentration and the acidity of the running buffer, separation voltage, injection time and detection potential were investigated to acquire the optimum conditions. The detection electrode was a 300 μm diameter carbon disc electrode at a working potential of +0.90 V (vs. Saturated Calomel Electrode (SCE)). The three analytes can be well separated within 10 min in a 50 cm length fused silica capillary at a separation voltage of 15 kV in a 50 mM borate buffer (pH 9.2). The relation between peak current and analyte concentration was linear over about 3 orders of magnitude with the detection limits (S/N = 3) ranging from 0.20 to 0.36 μM for all analytes. The proposed method has been successfully applied to the determination of the bioactive constituents in the real herbal samples with satisfactory assay results.]]>Baldwin, R.P.,20002140174028Chen, G., J.X. Zhang and J.N. Ye,2001923255262Chen, G., Q.C. Chu, L.Y. Zhang and J.N. Ye,2002457225233Chen, G., X.H. Han, L.Y. Zhang and J.N. Ye,2002954267276Chen, G., L.Y. Zhang, X.L. Wu and J.N. Ye,20055301521Chou, T.C.,200313911461152Chu, Q.C., L. Fu, Y.Q. Guan and J.N. Ye,20045278287833Deng, C.H., N. Yao, B. Wang and X.M. Zhang,200611031521Fliser, D., S. Wittke and H. Mischak,20052627082716Guan, Y.Q., T. Wu, M. Lin and J.N. Ye,2006Ipomoea batatas L.) by capillary electrophoresis with electrochemical detection.]]>542428Guo, L.B., J.J. Liang and Q.Y. Yang,1996Cynanchum paniculatum (Bge.) Kitag by gas chromatography.]]>21484485, 511Holland, L.A. and A.M. Leigh,20022336493658Huck, C.W., G. Stecher, H. Scherz and G. Bonn,20052613191333Jiang, S.P. and Y.X. Chen,199419311314Jorgenson, J.W. and K.D. Lukacs,19815312981302Jorgenson, J.W. and K.D. Lukacs,1981218209216Kim, S.H., S.A. Kim, M.K. Park, S.H. Kim and Y.D. Park et al.,20044279287Lin, C.C., Y.T. Li and S.H. Chen,20032441064115Peng, Y.Y., J.N. Ye and J.L. Kong,2005Perilla frutescens L. by capillary electrophoresis with electrochemical detection.]]>5381418147Piergiovanni, A.R. and G. Taranto,20055365936597Yu, F.,200528231232Zhan, W.Q. and H. Li,200427104105Zhang, L.Z.,1989Cynanchum paniculatum (Bunge) Kitag.]]>201518