Research Journal of Parasitology1816-4943xxxx-xxxxScience International10.3923/jp.2016.67.72HassanSoad E. Abou-El-DobalSanaa K.A. Abdel-RahmanEman H. 32016113Background and Objective:Trichostrongylus colubriformis is a common nematode which infect livestock and can cause accidental infection in humans. In the present study, affinity purification process succeeded in the isolation of a single specific fraction identified by experimentally infected rabbit sera. Methodology: The isolated fraction detected T. colubriformis antibodies in 68 (77.3%) out of 88 examined sheep serum samples. By SDS-PAGE, the isolated fraction showed simple electrophoretic profile and resolved into three bands of molecular weights 74, 85 and 97 kDa. While eight bands of molecular weights ranged from 25.7-189 kDa were detected in crude adult worm extract. In immunoblot assay, the three bands of isolated fraction were reacted with infected rabbit serum compared with four bands of molecular weights 41, 74, 85 and 97 kDa in crude extract. Results: Concerning, humeral immune responses of infected rabbits, the level of IgG antibodies increased to reach (0.526 OD±0.0027) in the first-week post infection. Then decreased to reach (0.34 OD±0.02) at the end of the experiment using ELISA. A significant expression of IL4 was observed and reached the highest level (225±0.012) at second-week post infection. While, IFN-ɣ showed less and nearly constant expression through out the time of the experiment. Conclusion: The partially immuno-affinity purified fraction of T. colubriformis adult antigen proved high diagnostic efficacy in diagnosis of trichostrongylosis in sheep. In addition, T. colubriformis infection stimulates rabbits cellular and humeral immune responses IL4, IFN-γ and IgG which, possibly create some level of protection to re infection.]]>Abou-El-Dobal S.K.A., S.E. Hassan, N.I. Toaleb, A.G. Hegazi and E.H. Abdel-Rahman,2015Fasciola gigantica excretory secretory products.]]>2015Allen, J.E. and R.M. Maizels,201111375388Anthony, R.M., L.I. Rutitzky, J.F. Urban Jr., M.J. Stadecker and W.C. Gause,20077975987Baerman, G. and R. Wetzal,19531st Edn.,Bezubik, B., H. Wedrychovicz and A. Wojciechowska,1988Trichostrongylus colubriformis in rabbits: Some quantitative aspects and pathogenesis of single and multiple infection.]]>33131142 Cardia, D.F.F., R.A. Rocha-Oliveira, M.H. Tsunemi and A.F.T. Amarante,2011Trichostrongylus colubriformis infections.]]>182248258Dynes, R.A., D.P. Poppi, G.K. Barrell and A.R. Sykes,1998794754Engvall, E. and P. Perlmann,19718871874Fabre, V., D.P. Beiting, S.K. Bliss, N.G. Gebreselassie and L.F. Gagliardo et al.,200918215771583Schmid-Hempel, P.,20093648598Laemmli, U.K.,1970227680685Langrova, I. and I. Jankovska,2004Trichostrongylus colubriformis in experimentally infected rabbits. Effect of decreasing photoperiod, low temperature and desiccation.]]>418590Lone, B.A., M.Z. Chishti, F. Ahmad, H. Tak and J. Hassan,2012Haemonchus contortus infection in sheep by indirect Enzyme Linked Immunosorbent Assay (ELISA).]]>134953Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275McLeod, R.S.,20042004pp: 23-33pp: 23-33Mulcahy, G., S. O'Neill, J. Fanning, E. McCarthy and M. Sekiya,200521273277Murphy, L., N. Nalpas, M. Stear and I.M. Cattadori,201133287302Murphy, L., A.K. Pathak and I.M. Cattadori,201335421432Razzaq, A., K. Ashraf, A. Maqbool, M. A. Khan, M. Islam and H. Khan,20132315591565Rocha, R.A., K.D.S. Bresciani, T.F.M. Barros, L.H. Fernandes, M.B. Silva and A.F.T. Amarante,200875135143Shaw, R.J., M.M. McNeill, D.R. Maass, W.R. Hein and T.K. Barber et al.,2003Trichostrongylus colubriformis.]]>3312331243Soulsby, E.J.,19866th Edn.,Tak, I.U.R., M.Z. Chishti and F. Ahmad,2015Haemonchus contortus found in sheep of Kashmir valley.]]>39639644Tan, T.K., C. Panchadcharam, V.L. Low, S.C. Lee, R. Ngui, R.S.K. Sharma and Y.A.L. Lim,2014Haemonchus contortus and Trichostrongylus spp. among livestock in Malaysia as revealed by amplification and sequencing of the internal transcribed spacer II DNA region.]]>2014Towbin, H., T. Staehelin and J. Gordon,19797643504354Waller, P.J. and P. Chandrawathani,2005Haemonchus contortus: Parasite problem No. 1 from tropics-polar circle. Problems and prospects for control based on epidemiology.]]>22131137Woo, P.C.Y., A.S.P. Leung, S.K.P. Lau and K.T.K. Chong,2001Aspergillus fumigatus-associated diseases.]]>3945984600Wray, W., T. Boulikas, V.P. Wray and R. Hancock,1981118197203Zhang, W.Y., E. Moreau, J.C. Hope, C.J. Howard, W.Y. Huang and A. Chauvin,2005Fasciola hepatica and Fasciola gigantica: Comparison of cellular response to experimental infection in sheep.]]>111154159