Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2018.205.210Channa micropeltes for Increasing Lymphocyte and Fibroblast Cells in Diabetic Wound Healing]]>Laillyza ApriasariMaharani PuspitasariDewi 42018184Background and Objective: Diabetes mellitus (DM) has the complications in the form of a wound healing process which take a long time. The DM patients of Indonesia have ranks 4th largest in the world. Empirically the people of South Kalimantan believe that Haruan fish (Channa striata) and Toman fish (Channa micropeltes) can accelerate wound healing. Both of them have the same genus that contain omega-6, albumin and zinc which can accelerate wound healing. To analyze the effect of toman fish extract orally at a dose of 16 mL kg1 of rat as alternative therapy of diabetic wound healing by increasing the number of lymphocyte and fibroblast cells on the 2nd, 4th and 8th days. Materials and Methods: This study was experimental with post test only control design. The sample used 36 male wistar rats, 200-250 mg weight as the model of diabetic wound healing on the back of skin. The study consisted of 3 groups: negative control (only fed), treatment (given toman fish extract 16 mL kg1) and positive control (given haruan fish extract 13.54 mL kg1) given for 2 times a day. On day 2, 4 and 8 rats were sacrificed and did the biopsy, then did the histopathology examination by Hematoxylin Eosin to see the number of lymphocytes and fibroblast cells. Results: Shapiro Wilk normality test (p>0.05) and Variance Levene's Homogeneity Test (p>0.05) obtained normal data distribution results and homogeneous data variance, then followed by LSD post hoc test. The highest mean value of the number of lymphocytes in the treatment group for the 4th days (15.20±0.84) and the lowest in the negative control group for the 2nd day (5.60±2.30). The highest mean value of the number of fibroblasts in the 4th day treatment group (24.20±1.79) and the lowest in the negative control group 2nd day (10.60±1.14). Conclusion: The administration of toman fish extract orally at a dose of 16 mL kg1 BB of rat can be alternative therapy of diabetic wound healing by increasing the highest lymphocyte and fibroblast cells on 4th day.]]>Mansur,2013Diabetes mellitus.]]>pp: 31pp: 31Kementerian Kesehatan,20132013Julianto, I.G.P., U. Elfiah and K.D. Sofiana,2015Merremia mammosa (Lour)) terhadap proses penyembuhan luka dan kadar gula darah pada tikus jantan hiperglikemi.]]>114Irwanda, W.F., M. Andrie and S. Luliana,2015Channa micropeltes) padda tikus putih jantan wistar yang diberi luka sayat.]]>11014Muslim and M. Syaifudin,2012Channa striata) Dalam Lingkungan Budidaya (Kolam Beton).]]>pp: 24pp: 24Gufran, H.M. and K. Kordi,2009Page: 152Page: 152Mustafa, A., M.A. Widodo and Y. Kristianto,2012Channa striata) extract and its role in health.]]>118Apriasari, M.L, A.N. Carabelly and D. Puspitasari,2018Channa micropeltes) in wound healing process.]]>148390Firlianty, S. Eddy, N. Happy, Hardoko and M. Annasari,201322531Nicodemus, M. Andrie and S. Luliana,2014Channa micropeltes) secara oral pada tikus putih jantan wistar.]]>1114Handaya, A.Y., Tepat and J. Atasi,2016pp: 29-31pp: 29-31Winarsi, H., S.P. Wijayanti and A. Purwanto,201244712Kumar, V., R.S. Cotran and S.L. Robbns,20107th Edn.,pp: 65-80pp: 65-80Cavalcante, G.M., R.J.S. de Paula, L.P. de Souza, F.B. Sousa, M.R.L. Mota and A.P.N.N. Alves,201126227234Omar, M.N., N.S.A.M. Yusoff, N.A. Zainuddin and A.M. Zuberdi,2014Channa micropeltes) fish oil.]]>3011331136Serhan, C.N., P.A. Ward and D.W. Gilroy,2010Page: 170Page: 170McCulloch, J.M. and L.C. Kloth,2010pp: 234pp: 234Kurahashi, T. and J. Fujii,201535770Akbik, D., M. Ghadiri, W. Chrzanowski and R. Rohanizadeh,201411617Rosenbaum, M.A., K. Miyazaki and L.M. Graham,201255489496Sayuti, K. and R. Yenrina,2015pp: 7-38pp: 7-38Izzati, A., N. Dewi and D.I.N. Pratiwi,2014Channa striata) decreases lymphocyte count in inflammatory phase of wound healing process effectively.]]>3176181Rifasanto, M.I., M.L. Apriasari and I. Taufiqurrahman,2018Musa acuminata) stem extract gel application with 37.5% concentration on fibroblast cell count. (In vivo study on wound healing process of male wistar rat (Rattus norvegicus) buccal mucosa).]]>316Apriasari, M.L., Y.P. Dachlan and D.S. Ernawati,201611135141