Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2018.164.171RevillaGusti AfrianiNita RusnitaDewi 42018184Background and Objective: Burn wound is a serious issue in health because of the outcome results the physical disability, influencing the morbidity of the patient, high cost of burn wound treatment. Treatment of full depth burn has been done in various ways by injecting growth factors and skin grafts, but the result has not provided satisfactory, its due to the formation of scar tissue, the absence of hair follicles and sebaceous tissue. Bone marrow stem cells (BM-MSCs) are strong potential cell in skin tissue regeneration. Study on BM-MSCs administration to burn wound is unclear and this study was performed to identify the formation of granulation tissue and to investigate the expression level of transforming growth factor beta 3 (TGF-β3) and matrix metalloproteinase-9 expression (MM-9). The TGF-β3 plays a role in reducing scar tissue formation and MMP9 serves as collagen destruction. Materials and Methods: Ten Wistar rats were divided into two groups, i,e., control group was treated with PBS and treated group was injected with BM-MSCs. A burn wound was made on dorsal area of rat. On day 14 after treatment, burn wound was investigated; immunohistochemical examination was performed to identify granulation tissue formation, TGF-β3 and MMP-9 expression. Results: The administration of BM-MSCs on day 14 accelerated the burns healing in rat and increased the expression level of TGF-β3 (7.32) and MMP-9 (6.26) significantly, compared with in control group (2.64 and 3.98). Conclusion: The BM-MSCs accelerated the burns healing in rat by elevating the expression level of TGF-β3 and MMP-9 which acts in proliferation phase in wound healing.]]>Pittenger, M.F., A.M. Mackay, S.C. Beck, R.K. Jaiswal and R. Douglas et al.,1999284143147Rohban, R. and T.R. Pieber,20172017Cerqueira, M.T., R.P. Pirraco and A.P. Marques,20165164175Isakson, M., C. De Blacam, D. Whelan, A. McArdle and A.J.P. Clover,20152015Sasaki, M., R. Abe, Y. Fujita, S. Ando, D. Inokuma and H. Shimizu,200818025812587Kirby, G.T.S., S.J. Mills, A.J. Cowin and L.E. Smith,20152015Li, D.J., C.A. Shen, T.J. Sun, L. Zhang, H.P. Deng and J.K. Chai,20171613171323Chen, L., Y. Xu, J. Zhao, Z. Zhang and R. Yang et al.,20142014Hu, C., X. Yong, C. Li, M. Lu and D. Liu et al.,2013183427434Wu, Y., L. Chen, G. Scott and E.E. Tredget,20072526482659Oswald, J., S. Boxberger, B. Jorgensen, S. Feldmann, G. Ehninger, M. Bornhauser and C. Werner,2004in vitro.]]>22377384Smith, A.N., E. Willis, V.T. Chan, L.A. Muffley, F.F. Isik, N.S. Gibran and A.M. Hocking,20103164854Al-Khaldi, A., H. Al-Sabti, J. Galipeau and K. Lachapelle,200375204209Salvolini, E., G. Lucarini, A. Zizzi, M. Orciani, G. Di Benedetto and R. Di Primio,2010302367374Le, M., R. Naridze, J. Morrison, L.C. Biggs and L. Rhea et al.,2012in vivo.]]>2012Stavri, G.T., Y. Hong, I.C. Zachary, G. Breier and P.A. Baskerville et al.,1995358311315Bode, W. and K. Maskos,2003384863872Jinnin, M., H. Ihn, Y. Mimura, Y. Asano, K. Yamane and K. Tamaki,2005202510517Bove, P.F., U.V. Wesley, A.K. Greul, M. Hristova, W.R. Dostmann and A. van der Vliet,200536138146Paramonov, V.A. and V.Y. Chebotarev,2002134512515Kumar, S., P. Wong and D.J. Leapar,200434147160Singer, A.J. and R.A.F. Clark,1999341738746Shumakov, V.I., N.A. Onishchenko, M.F. Rasulov, M.E. Krasheninnikov and V.A. Zaidenov,2003136192195Rasulov, M.F., A.V. Vasil'chenkov, N.A. Onishchenko, M.E. Krasheninnikov and V.I. Kravchenko et al.,2005139141144Hao, L., J. Wang, Z. Zou, G. Yan and S. Dong et al.,2009163442Singer, D.D., A.J. Singer, C. Gordon and P. Brink,201320398402Liu, L., Y. Yu, Y. Hou, J. Chai and H. Duan et al.,20142014Yang, Y., W. Zhang, Y. Li, G. Fang and K. Zhang,201426289295Revilla, G., E. Darwin and F.A. Rantam,201619345351Shah, M., D.M. Foreman and M. Ferguson,19951089851002LeBert, D.C., J.M. Squirrell, J. Rindy, E. Broadbridge and Y. Lui et al.,201514221362146Li, W.W., D. Tsakayannis and V.W. Li,20031511Leong, M. and L.G. Phillips,20122012pp: 151-160pp: 151-160Chen, L., E.E. Tredget, P.Y.G. Wu and Y. Wu,20082008Wu, Y., R.C. Zhao and E.E. Tredget,201028905915Gilbert, R.W.D., M.K. Vickaryous and A.M. Viloria-Petit,20162016Lee, P.T. and W.J. Li,2017118172181Qi, Y., D. Jiang, A. Sindrilaru, A. Stegemann and S. Schatz et al.,2014134526537Jiang, F., J. Ma, Y. Liang, Y. Niu, N. Chen and M. Shen,2015in vitro and in vivo.]]>2015Logsdon, E.A., S.D. Finley, A.S. Popel and F.M. Gabhann,20141814911508