Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2018.134.142In vitro Study]]>Abdel Naser Abdel RahmanGehan Mostafa El-AzabSamia Helmy El BolokAmr Farouk El-GayarSherif Fahmy MohamedAly 32018183Background and Objective: Sinapic acid is a cinnamic acid derivative that widely distributed in plant kingdom such as in various fruits, vegetables, cereal grains, oilseed crops, some spices and medicinal plants. Sinapic acid act as preventive and therapeutic agents in several oxidative stress related diseases, such as atherosclerosis, inflammatory injury and cancer. The current study aimed to examine cytotoxicity of free and Nano-sinapic acid on squamous cell carcinoma (HEp-2). Material and Methods: The present work conducted to evaluate the cytotoxicity using MTT assay, evaluation of morphological changes post cell treatment with free and Nanocapsulated Sinapic acid. Nanocapsulation was performed using Tween-80, also evaluation of cell apoptosis and related pro and anti apoptotic genes using rt-PCR concerning (Bax and BCl-2) and cell apoptosis process. Finally, geno-toxicity was traced via arranging for flow cytometry. Results: Data recorded revealed that the inhibitory concentration (IC50) of free and nanocapsulated sinapic acid was 646.4 μM mL1 and 84.74 μM mL1 post treatment with free and nanocapsulated sinapic acid, respectively. Also, cytotoxicity was concentration dependent with significant difference between free and capsulated one (p<0.05). Cytotoxicity enhanced apoptosis process accompanied with cell cycle arrest through the induction of G2/M and pre-G1 phases arrest compared with that of cell control. Finally, proapoptotic gene (Bax) was significantly upregulated parallel with significant down regulation of BCL-2 (p<0.05). Conclusion: Current study suggested that Nano-sinapic acid possesses more cytotoxic effect on cancer cells with lower concentration than free sinapic acid so can act better as novel candidate for cancer chemoprevention.]]>WHO.,20082008Thun, M.J., J.O. DeLancey, M.M. Center, A. Jemal and E.M. Ward,200931100110Saxena, A., A.K. Saxena, J. Singh and S. Bhushan,2010Cedrus deodara in human leukemia HL-60 cells.]]>188580590Li, J.W. and J.C. Vederas,201157148160, (In Russian)Mansoori, G.A., P. Mohazzabi, P. McCormack and S. Jabbari,20074226257Shahidi, F. and M. Naczk,20042004pp: 117‐166pp: 117‐166Kwak, S.Y., J.K. Yang, H.R. Choi, K.C. Park, Y.B. Kim and Y.S. Lee,20132311361142Mosmann, T.,1983655563Chakraborty, D., A. Samadder, S. Dutta and A.R. Khuda-Bukhsh,2012Helonias dioica: Antioxidative stress responses and the signaling cascade.]]>2376476Niciforovic, N. and H. Abramovic,2014133451Reddy, B.V. and N.R. Prasad,20116743755Kern, S.M., R.N. Bennett, F.A. Mellon, P.A. Kroon and M.T. Garcia-Conesa,20035160506055Hudson, E.A., P.A. Dinh, T. Kokubun, S. Monigue, J. Simmonds and G. Andreas,2000911631170Cheng, J.C., F. Dai, B. Zhou, L. Yang and Z.L. Liu,2007104132139Kampa, M., V.I. Alexaki, G. Notas, A.P. Nifli and A. Nistikaki et al.,20046R63R74Cragg, G.M. and D.J. Newman,20051007279Pommier, Y., O. Sordet, S. Antony, R.L. Hayward and K.W. Kohn,20042329342949Janakiraman, K., S. Kathiresan and A.V. Mariadoss,20142165170Rugina, D., S. Vicas, M. Petran, A. Pintea, A. Bunea and C. Socaciu,201067429436Bartek, J. and J. Lukas,2001490117122Chen, H.W. and H.C. Huang,199812410291040Kim, J.A., C. Aberg, A. Salvati and K.A. Dawson,201276268Daroui, P., S.D. Desai, T.K. Li, A.A. Liu and L.F. Liu,20042791458714594Barnes, D.M., C.E. Gillett, L.G. Bobrow, A.M. Hanby and S. Mohammed et al.,1992340259263Puangpraphant, S., M.A. Berhow and E.G. de Mejia,2011Ilex paraguariensis St. Hilaire) saponins induce caspase-3-dependent apoptosis in human colon cancer cells in vitro.]]>12511711178Prasad, S., N. Kalra, S. Srivastava and Y. Shukla,2008275563Hu, X., K. Bardhan, A.V. Paschall, D. Yang and J.L. Waller et al.,20132881910319115Rajasekaran, D., S. Manoharan, S. Silvan, K. Vasudevana, N. Baskaran and D. Palanimuthu,201310102112Chang, W.H., J.J. Liu, C.H. Chen, T.S. Huang and F.J. Lu,200243214226Halliwell, B. and J.M.C. Gutteridge,19892nd Edn.,pp: 96-98pp: 96-98