Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2016.7.15KhajehahmadiZohreh MehrabaniDavood Javad AshrafMohammad RahmanifarFarhad TanidehNader TamadonAmin ZareShahrokh 1-22016161-2Following repeated injury, the liver undergoes tissue remodeling and fibrosis. This study investigated the healing effect of conditioned media (secreta) from rat bone marrow-derived stem cells (BMSCs) in thioacetamide (TA)-induced liver fibrosis rat model. During 2014, thirty male Wistar rats were randomly allocated into two groups of sham control and treatment. The rats of sham control group were subdivided into three subgroups and were sampled on weeks 14, 18 and 20 after fibrosis induction. The rats of treatment group were subdivided into two subgroups and were sampled on weeks 4 and 6 after treatment by conditioned media. Fibrosis was induced by intraperitoneal administration of TA. Animals were valuated histologically and for liver function tests after TA-induced fibrosis and injection of conditioned media. Conditioned media were provided from the supernatant of BMSCs culture flasks. Administration of TA resulted into infiltration of inflammatory cells and deposition of collagen fibers in hepatic tissue. A significant healing was noticed in liver four and six weeks after post-injection of BMSCs conditioned media. Regarding fibrosis and necrosis, a significant reduction appeared 4 and 6 weeks after BMSCs conditioned media therapy. Aspartate aminotransferase (AST) and alanine transaminase (ALT) levels decreased 4 and 6 weeks after BMSCs conditioned media therapy. Regarding alkaline phosphatase (ALP) and albumin levels 4 and 6 weeks after BMSCs conditioned media therapy, a non-significant increase was seen. This study indicated that the BMSCs conditioned media had a positive healing effect in rearrangement of hepatic fibrosis. So it can be an alternative therapeutic choice to ameliorate liver fibrosis.]]>Abdel Aziz, M.T., H.M. Atta, S. Mahfouz, H.H. Fouad and N.K. Roshdy et al.,200740893899Ahmed, S.K., S.A. Mohammed, G. Khalaf and H. Fikry,20144 induced liver fibrosis in albino rats: A histological and immunohistochemical study.]]>78797Ai, J., S. Ebrahimi, A. Khoshzaban, T.S.J. Kashi and D. Mehrabani,20121496103Arthur, M.J.P.,200212215251528Ayatollahi, M., Z. Hesami, A. Jamshidzadeh and B. Gramizadeh,20145166173Chilakapati, J., K. Shankar, M.C. Korrapati, R.A. Hill and H.M. Mehendale,20053318771885Davarpanah, M.A., M. Saberi-Firouzi, K.B. Lankarani, D. Mehrabani and A. Behzad-Behbahani et al.,20099122127Dehghani, S.M., S. Gholami, A. Bahador, M. Haghighat and M.H. Imanieh et al.,20073931753177Fang, B., M. Shi, L. Liao, S. Yang, Y. Liu and R.C. Zhao,2004+ mesenchymal stem cells ameliorate carbon tetrachloride-induced liver fibrosis in mice.]]>158388Farjam, M., D. Mehrabani, F. Abbassnia, N. Tanideh and M.H. Imanieh et al.,2014Curcuma longa on liver in experimental acute hepatic encephalopathy of rat.]]>2316691673Ghobadi, F., D. Mehrabani and G. Mehrabani,2015438Gnecchi, M., H. He, O.D. Liang, L.G. Melo and F. Morello et al.,200511367368Haghighi, R.N., N. Naghsh and D. Mehrabani,2013Curcuma longa in thioacetamide-induced hepatic injury in rat.]]>7203207Hosseinkhani, M., D. Mehrabani, M.H. Karimfar, S. Bakhtiyari, A. Manafi and R. Shirazi,2014337Jang, Y.O., M.Y. Kim, M.Y. Cho, S.K. Baik, Y.Z. Cho and S.O. Kwon,20142014Katoonizadeh, A., H. Poustchi and R. Malekzadeh,20143414641472Malekzadeh, R., M. Mohamadnejad, K. Alimoghaddam, M. Bagheri, H. Baharvand and A. Ghavamzadeh,201012127Mehrabani, D., G. Mehrabani, S. Zare and A. Manafi,201326570Mohamadnejad, M., K. Alimoghaddam, M. Mohyeddin-Bonab, M. Bagheri and M. Bashtar et al.,200710459466Mostaghni, A.A., A. Soltanian, E. Mokhtari, S. Japoni and D. Mehrabani,201111200202Nikeghbalian, S., B. Pournasr, N. Aghdami, A. Rasekhi and B. Geramizadeh et al.,2011+ cells in patients with decompensated cirrhosis.]]>141217Okura, H., M. Soeda, M. Morita, M. Fujita and K. Naba et al.,2014456860865Ortiz, L.A., M. DuTreil, C. Fattman, A.C. Pandey, G. Torres, K. Go and D.G. Phinney,20071041100211007Pournasr, B., M. Mohamadnejad, M. Bagheri, N. Aghdami, M. Shahsavani, R. Malekzadeh and H. Baharvand,2011In vitro differentiation of human bone marrow mesenchymal stem cells into hepatocyte-like cells.]]>14244249Qi, X., X. Guo and C. Su,201510166180Tang, Y.L., Q. Zhao, Y.C. Zhang, L. Cheng and M. Liu et al.,2004117310Togel, F., K. Weiss, Y. Yang, Z. Hu, P. Zhang and C. Westenfelder,2007292F1626F1635Wang, Y., X. Nan, Y. Li, R. Zhang, W. Yue, F. Yan and X. Pei,20052m-c‐Met+ cells into hepatocyte‐like cells by coculture with CFSC/HGF cells.]]>11635643