Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2011.77.83In-vivo Oxidative DNA damage, Protein Oxidation and Lipid Peroxidation as a Biomarker of Oxidative stress in Preterm Low Birth Weight Infants]]>NegiReena PandeDeepti KumarAshok BasuSriparna KhannaRanjana S.KhannaHari D.22011112Pregnancy while not a disease is often accompanied by a high energy demand of many bodily functions. Neonates are said to be more susceptible to oxygen radical injury. The objective of the present study was to measure cord blood levels of 8-OHdG, malondialdehyde, protein carbonyl and total antioxidant status in premature low birth weight infants to analyze the status of oxidative stress in relation to the degree of prematurity and birth weight of neonates. Umbilical cord blood samples were obtained at the time of delivery. 8-OHdG has been measured as oxidative DNA damage marker in preterm LBW newborns by competitive in vitro Enzyme-Linked Immunosorbent Assay (ELISA) along with malondialdehyde as marker of lipid peroxidation, protein carbonyl as marker of protein oxidation and total antioxidant status to study the oxidative stress. Significant elevation in the levels of 8-OHdG along with malondialdehyde, protein carbonyl has been noted in preterm LBW newborns. Serum 8-OHdG is found to be significantly and negatively correlated with birth weight (r = -0.834, p<0.001) and gestational age of the newborn (r = -0.626, p<0.001). These results provide evidence of increased oxidative stress in the form of DNA damage, protein oxidation and lipid peroxidation in premature LBW newborns which may be responsible for different complications associated with prematurity.]]>Ames, B.N., M.K. Shigenaga and T.M. Hagen,19939079157922El-Bassyouni, H.T., A.M. Ashour, A. Ezzat, R. Bassiouni and E.M. Fateen,20066452457Trindade, C.E.P. and L.M.S.S. Rugolo,20078522532Del Rio, D., A.J. Stewart and N. Pellegrini,200515316328Demple, B. and L. Harrison,199463915948Halliwell, B. and J.M.C. Gutteridge,19993rd Edn.,pp: 105-350pp: 105-350Halliwell, B. and J.M.C. Gutteridge,1990186185Hill, H.B.,197910th Edn.,pp: 132pp: 132Inder, T.E., P. Graham, K. Sanderson and B.J. Taylor,199470107111Inoue, T., M. Hayashi, K. Takayanagi and S. Morooka,200349217220Ishikawa, T., H. Fujioka, T. Ishimura, A. Takenaka and M. Fujisawa,2007100863866Kelly, F.J.,199349668678Levine, R.L., D. Garland, C.N. Oliver, A. Amici and I. Climent et al.,1990186464478Mahmoud, A.H., H.M. Motawa, H.E. Wahba and A.Y. Ebrahim,2006Urtica pilulifera extracts.]]>16774Marjani, A., A. Moradi and A.B. Ghourcaie,20072118123Marjani, A., A.R. Mansourian, G.R. Veghari and M.R. Rabiee,2007717951799Matsubasa, T., T. Uchino, S. Karashima, M. Tanimura and F. Endo,200236189193McCord, J.M.,2000108652659Al-Menabbawy, K., M. Sallam, S. Taha, H. Mottawie and A. Ibrahiem,20066956961Miller, N.J., C. Rice-Evans, M.J. Davies, V. Gopinathan and A. Milner,199384407412Nassi, N., V. Ponziani, M. Becatti, P. Galvan and G. Donzelli,200951183187Okur, H., M. Kucukaydin, K. Kose, O. Kontas, P. Doan and A. Kazez,19953014161419Philpot, J.St.L.,196335570Perrone, S., P. Vezzosi, M. Longini, B. Marzocchi and P. Paffetti et al.,20091547552Pitkanen, O.M., M. Hallman and S.M. Anderson,1990116760764Richter, C., J.W. Park and B.N. Ames,19888564656467Rogers, M.S., W. Wang, M. Mongelli, C.P. Pang, J.A. Duley and A.M.Z. Chang,199744229233Saker, M., N.S. Mokhtari, S.A. Merzouk, H. Merzouk, B. Belarbi and M. Narce,20081419599Saugstad, O.D.,19968514Saugstad, O.D.,19978612771282Saugstad, O.D.,200113147153Scholl, T.O. and T.P. Stein,200110182185Shigenaga, M.K. and B.N. Ames,1991in vivo oxidative DNA damage.]]>10211216Shoji, H., S. Oguchi, T. Shimizu and Y. Yamashiro,200353850852Balkis, B.S., R. Nor Fadilah, A. Ezlan, O. Khairul, A.B. Mokhtar and M. Jamaludin,20088622627Uma Devi, P. and P. Chinnaswamy,20083143152UNICEF,2003Winterbourn, C.C., T. Chan, I.H. Buss, T.E. Inder, N. Mogridge and B.A. Darlow,2000488490Yin, B., R.M. Whyatt, F.P. Perera, M.C. Randall, T.B. Cooper and R.M. Santella,19951810231032