Journal of Medical Sciences1682-44741812-5727Asian Network for Scientific Information10.3923/jms.2007.361.367Toxoplasma gondii SAG1-Encoded DNA Vaccine by Formulation with Aluminum Phosphate]]>SolhjooK. Ghaffari FarF. Dalimi-AslA. SharifiZ. 3200773In the present study, we evaluated aluminum phosphate as an adjuvant of SAG1-encoded DNA vaccine against T. gondii in BALB/c mice. Anti-T. gondii IgG values increased markedly in the pcSAG1 and pcSAG1+AlPO4 groups, which were significantly higher than those of control groups (p< 0.05). Although pcSAG1+AlPO4 elicited IgG antibody values were greater than that of the sera of mice immunized with pcSAG1 alone, there was no statistically significant difference between these two groups (p>0.05). In spite of remaining alive of one mouse more than 45 days in pcSAG1+AlPO4 group after challenge with T. gondii tachyzoites, all of the experimental mice died and the survival time of the mice immunized with pcSAG1+AlPO4 was markedly longer than those of control and pcSAG1 groups (p< 0.05). All mice in the control groups died within 7-9 days after challenge, whereas the average survival time of pcSAG1 and pcSAG1+AlPO4 groups were up to 14 and 21 days after infection, respectively. Indeed its low cost, ease of use and safety record, these findings indicate that the AlPO4 adjuvant can be used to combine DNA-based vaccination to prime an enhanced and balanced specific immunity.]]>Bhopale, G.M.,20035457462Biemans, R., D. Gregoire, M. Haumont, A. Bosseloir, L. Garcia, A. Jacquet, C. Dubeaux and A. Bollen,1998Toxoplasma gondii SAG1 antigen, secreted from engineered Pichia pastoris, is adequate for serorecognition and cell proliferation.]]>66137146Bonenfant, C., I. Dimier-Poisson, F. Velge-Roussel, D. Buzoni-Gantel, G.D. Giudice, R. Rappuoli and D. Bout,2001Toxoplasma gondii.]]>6916051612Brewer, J.M., M. Conacher, C.A. Hunter, M. Mohrs, F. Brombacher and J. Alexander,199916364486454Bulow, R. and J.C. Boothroyd,1991Toxoplasma gondii infection by immunization with p30 antigen in liposomes.]]>14734963500Burg, J.L., D. Perelman, L.H. Kasper, P.L. Ware and J.C. Bothroyd,1988Toxoplasma gondii.]]>14135843591Chen, X.G., Y. Gong, Hua-Li, Z-R. Lun and M-C. Fung,2001Toxoplasma gondii in Escherichia coli.]]>233337Couper, K.N., H. Nielsen, E. Petersen, F. Roberts, C.W. Roberts and J. Alexander,2003Toxoplasma gondii infection but does not prevent maternofoetal transmission.]]>2128132820Crowther, J.R.,1995Debard, N., D. Buzoni-Gatel and D. Bout,1996Toxoplasma gondii in association with cholera toxin dramatically reduces development of cerebral cysts after oral infection.]]>6421582166Fachado, A., A. Rodriguez, S.O. Angel, D.C. Pinto and I. Vila et al.,20032113271335Fischer, L., J. Minke, N. Dufay, P. Baudu and J.C. Audonnet,20032145934596Guanjin, C., C. Haifeng, G. Hong and Z. Huanqin,2002Toxoplasma gondii in mice.]]>11514481452Gupta, R.K. and B.E. Rost,20002000pp: 65-89pp: 65-89Chen, H., G. Chen, H. Zheng and H. Guo,2003Toxoplasma gondii SAG1 and ROP1 genes.]]>11615611566Kasper, L.H., K.M. Currie and M.S. Bradley,1985Toxoplasma gondii.]]>13434263431Kimbita, E.N., X. Xuan, X. Huang, T. Miyazawa and S. Fukumoto et al.,2001Toxoplasma gondii infection in cats by enzyme-linked immunosorbent assay using recombinant SAG1.]]>1023544Kwissa, M., E.B. Lindblad, R. Schirmeck and J. Reimann,200381502510Laemmli, U.K.,1970227680685Letscher-Bru, V., O. Villard, B. Risse, M. Zauke, J-P. Klein and T.T. Kien,19986645034506Letscher-Bru, V., A.W. Pfaff, A. Abou-Bacar, D. Filisetti and E. Antoni et al.,2003Toxoplasma gondii SAG-1 Protein Is Protective against congenital Toxoplasmosis in BALB/c Mice but Not in CBA/J Mice.]]>7166156619Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Martin, V., M. Arcavi, G. Santillan, M.R. Amendoeira and E. De Souza Neves et al.,1998Toxoplasma gondii ROP2 protein.]]>5627631Meek, B., R.J. Diepersloot, T.V. Gool, D. Speijer and R. Peek,2003Toxoplasma gondii antigens by sera acutely or latently infected humans.]]>454552Mohamed, R.M., F. Aosai, M. Chen, H.S. Mun and K. Norose et al.,2003Toxoplasma gondii HSP70, HSP30 and SAG1 genes.]]>2128522861Nielsen, H.V., S.L. Lauemoller, L. Christiansen, S. Buus, A. Fomsggard and E. Petersen,1999Toxoplasma gondii infection in mice immunized with a plasmid encoding the SAG1 gene.]]>6763586363Nigro, M., A. Gutierrez, A.M. Hoffer, M. Clemente and F. Kaufer et al.,2003Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired Toxoplasmosis by an immunoglobulin G analysis.]]>47609613Petersen, E., H.V. Nielsen, L. Christiansen and J. Spenter,1998E. coli produced recombinant T. gondii SAG1 with alum as adjuvant protect mice against lethal infection with Toxoplasma gondii.]]>1612831289Rojas, P.A., V. Martin, M. Nigro, P.C. Echeverria, E.A. Guarnera, V. Pszenny and S.O. Angel,2000Toxoplasma gondii protein belonging to the heat-shock 90 family and analysis of its antigenicity.]]>190209213Rosado-Vallado, M., E. Dumonteil, R. Garcia-Miss Mdel and E. Dumonteil,20052353725379Saito, S., F. Aosai, N. Rikihisa, H. Mun and K. Norose et al.,2001Toxoplasma gondii infction in mice.]]>1921722180Sambrook, J., E.F. Fritsch and T.A. Maniatis,19892nd Edn.,Pages: 397Pages: 397Sasaki, S., F. Takeshita, K-Q. Xin, N. Ishii and K. Okuda,200331234254Temperton, N.J., D.C. Quenelle, K.M. Lawson, J.N. Zuckerman, E.R. Kern, P.D. Griffiths and V.C. Emery,2003708690Ulmer, J.B., C.M. Dewitt, M. Chastain, A. Friedman and J.J. Donnelly et al.,1999181828Velge-Roussel, F., P. Marcelo, A.C. Lepage, D. Buzoni-Gatel and D. Bout,2000Toxoplasma gondii SAG1 induces protective cells into NALT and GALT compartments.]]>68969972Wang, S., X. Liu, K. Fisher, J.Z. Smith, F. Chen, T.W. Tobery, J.B. Ulmer, R.K. Evans and M.J. Caulfield,20001812271235Zinecker, C.F., B. Striepen, H. Geyer, R. Geyer, J.F. Dubremetz and R.T. Schwarz,2001Toxoplasma gondii.]]>116127135